Clozapine (CLO) is an atypical antipsychotic drug indicated for the treatment of schizophrenia. The treatment effectiveness of CLO is better than that of other atypical antipsychotics, and it has the advantage of being able to determine its effectiveness by measuring its concentration in the patient\'s blood. Thus, sensitive, selective, and accurate determination of CLO in blood is highly significant for treatment monitoring. This study describes the design and fabrication of a molecularly imprinted polymer (MIP)-based electrochemical sensor for CLO determination. This is the first MIP-based electrochemical application in the literature for CLO determination. Employing the thermal polymerization approach, the MIP was formed on the glassy carbon electrode (GCE) using CLO as the template, trans-3-(3-Pyridyl)acrylic acid (3,3-TA) as the functional monomer, and the support of zinc oxide nanoparticles (ZnO NPs). Elaborate characterizations in terms of surface morphology and electrochemistry were performed via scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) methods. An indirect approach was employed to determine CLO in standard solution, real human biological samples, and tablet formulation, using 5 × 10-3 M [Fe(CN)6]3-/4- solution as the redox probe. The limit of detection (LOD) values for the standard solution and serum sample were calculated as 2.9 × 10-11 M and 6.01 × 10-12 M, respectively. These values and recovery studies confirmed the sensor\'s sensitivity and feasibility. The measurements in the presence of similarly structured compounds (olanzapine and quetiapine fumarate) verified the sensor\'s superior selectivity. Moreover, the developed sensor\'s performance was compared and verified using an LC-MS/MS method using the student\'s t-test and F-test.

Hg2+ is a highly toxic heavy metal ion that poses serious risks to human health and the environment. Due to its tendency to accumulate, it can easily enter the human body through the food chain, making it crucial to develop detection sensors that mimic real environmental conditions. To achieve this, our study employed a surface-enhanced Raman scattering (SERS) sensor using two strategies. First, we designed a highly selective probe by optimizing the probe and reporter DNA strands to bind Hg2+ within a thymine-thymine mismatch. Second, we used the double coffee ring effect to concentrate the optimized probe DNA. These two strategies greatly enhanced the SERS signal, resulting in a sensor with exceptional sensitivity, a low detection limit of 208.71 fM, and superior selectivity for Hg2+. The practical application of the sensor was demonstrated by successfully detecting Hg2+ in drinking water, tap water, canned tuna, and tuna sashimi. Additionally, the experimental results were presented in a pizza-shaped SERS mapping image, allowing users to estimate Hg2+ concentrations through color, providing a user-friendly and intuitive method for data comprehension and analysis. Our study presents a promising approach for sensitive and reliable Hg2+ detection, with potential implications for environmental monitoring and food safety.

This study presents a facial and quick electrochemical sensor platform that offers remarkable water and food safety applications. The present work represents a study of the synthesis and characterization for efficient cerium vanadate (CeVO4) with a functionalized carbon nanofiber (f-CNF) decorated electrode, which is a highly effective electrode modifier for sensitive nitrite detection. The CeVO4 nanoparticles were synthesized using the facial hydrothermal technique, and a composite (CeVO4@f-CNF) was prepared using the sonication method. Afterward, the produced materials were confirmed with spectroscopic and microscopic analysis. The electrochemical behavior of nitrite was studied through cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The DPV analysis depicts an excellent linear range of 0.1-1033 μM and a promising detection limit of 0.004 μM for the proposed electrode. The CeVO4@f-CNF electrode was applied to detect nitrite in water and meat samples. The proposed electrochemical sensor attributes the significant results towards the detection of nitrite.

This research explores the fluorescence properties and photostability of boron nitrogen co-doped graphene quantum dots (BN-GQDs), evaluating their effectiveness as sensors for rutin (RU). BN-GQDs are biocompatible and exhibit notable absorbance and fluorescence characteristics, making them suitable for sensing applications. The study utilized various analytical techniques to investigate the chemical composition, structure, morphology, optical attributes, elemental composition, and particle size of BN-GQDs. Techniques included X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM). The average particle size of the BN-GQDs was determined to be approximately 3.5 ± 0.3 nm. A clear correlation between the emission intensity ratio and RU concentration was identified across the range of 0.42 to 4.1 μM, featuring an impressively low detection limit (LOD) of 1.23 nM. The application of BN-GQDs as fluorescent probes has facilitated the development of a highly sensitive and selective RU detection method based on Förster resonance energy transfer (FRET) principles. This technique leverages emission at 465 nm. Density Functional Theory (DFT) analyses confirm that FRET is the primary mechanism behind fluorescence quenching, as indicated by the energy levels of the lowest unoccupied molecular orbitals (LUMOs) of BN-GQDs and RU. The method\'s effectiveness has been validated by measuring RU concentrations in human serum samples, showing a recovery range between 97.8% and 103.31%. Additionally, a smartphone-based detection method utilizing BN-GQDs has been successfully implemented, achieving a detection limit (LOD) of 49 nM.

In this work, we developed a fast and straightforward colorimetric and photoluminescent chemosensor probe (P1), featuring bis-thiophene-thiosemicarbazide moieties as its signaling and binding unit. This probe exhibited rapid sensitivity to Hg2+ and Cu2+ ions in a semi-aqueous medium, resulting in distinct colorimetric and photoluminescent changes. In the presence of Cu2+, P1 displayed an impressive 50-fold increase in photoluminescence (PL) at 450 nm (with excitation at 365 nm). The probe P1 formed a 1:1 complex with Hg2+ and Cu2+ ions, featuring association constant values of 4.04 × 104 M-1 and 1.25 × 103 M-1, respectively. P1 has demonstrated its efficacy in the analysis of real samples, yielding promising results. Additionally, the probe successfully visualized copper ions on a mouse fibroblast cell line (NIH3T3), highlighting its potential as an intracellular probe for copper ion detection.

A dyad Carbazolyl-bis(hydrazinobenzothiazole) was designed to form a symmetrical structure that containing two-arm active binding sites facilitates coordination with Hg2+ ion. This sensor has imparted a colorimetric and fluorometric changes in presence of Hg2+ ions. The ligand showed a selective blue shift in presence of Hg2+even in co-existence with heavy metal ions with luminescence change from colorless to blue and colorless to green under day light. Enhanced Intramolecular charge transfer process is responsible for fluorescence transformation when ligand interacts with Hg2+ ion. The emission spectra showed a ratiometric response to increasing addition of Hg2+ ions. The sensor is capable of detecting above the lower concentration of 6.8025×10-8 M. The fluorescence efficiency of CBT-2 with Hg2+ ion is quite stable under different co-metal ions and wide range of pH 6 to 9. The sensor CBT-2 forms a 1 : 1 stoichiometric complex with Hg2+ ions and the binding nature is confirmed from the 1H-NMR, FTIR, and mass spectroscopic studies. The sensor CBT-2 and its Hg2+ complex possess good binding nature to protein in Bovine Serum Albumin which could be good in biological applications. Additionally, wedevelop a practical application in real water sample analysis and electrochemical detection via oxidation potential discrimination.

A Schiff-base Ethyl (E)-2-(3-((2-carbamothioylhydrazono)methyl)-4-hydroxyphenyl)-4-methylthiazole-5-carboxylate (TZTS) dual functional colorimetric and photoluminescent chemosensor which includes thiazole and thiosemicarbazide has been synthesized to detect arsenic (As3+) ions selectively in DMSO: H2O (7:3, v/v) solvent system. The molecular structure of the probe was characterized via FT-IR, 1H, and 13C NMR & HRMS analysis. Interestingly, the probe exhibits a remarkable and specific colorimetric and photoluminescence response to As3+ ions when exposed to various metal cations. The absorption spectral changes of TZTS were observed upon the addition of As3+ ions, with a naked eye detectable color change from colorless to yellow color. Additionally, the chemosensor (TZTS) exhibited a new absorption band at 412 nm and emission enhancements in photoluminescence at 528 nm after adding As3+ ions. The limit of detection (LOD) for As3+ ions was calculated to be 16.5 and 7.19 × 10-9 M by the UV-visible and photoluminescent titration methods, respectively. The underlying mechanism and experimental observations have been comprehensively elucidated through techniques such as Job\'s plot, Benesi-Hildebrand studies, and density functional theory (DFT) calculations. For practical application, the efficient determination of As3+ ions were accomplished using a spike and recovery approach applied to real water samples. In addition, the developed probe was successfully employed in test strip applications, allowing for the naked-eye detection of arsenic ions. Moreover, fluorescence imaging experiments of As3+ ions in the breast cancer cell line (MCF-7) demonstrated their practical applications in biological systems. Consequently, these findings highlight the significant potential of the TZTS sensor for detecting As3+ ions in environmental analysis systems.

Heavy metals like Cadmium, Lead, and Chromium are the pollutants emitted into the environment through industrial development. In this work, a new diphenylamine coordinated cobalt complex (Co-DPA) has been synthesized and tested for its efficiency in removing heavy metals from wastewater, and its adsorption capacity was investigated. The effectiveness of heavy metals removal by Co-DPA was evaluated by adjusting the adsorption parameters, such as adsorbent dose, pH, initial metals concentration, and adsorption period. Heavy metal concentrations in real sample were 0.267, 0.075, and 0.125 mg/L for Cd2+, Pb2+, and Cr3+ before using as-synthesized Co-DPA to treat wastewater. After being treated with synthesized Co-DPA the concentration of heavy metals was reduced to 0.0129, 0.00028, 0.00054 mg/L for Cd2+, Pb2+, and Cr3+, respectively, in 80 min. The removal efficiency was 95.6%, 99.5%, and 99.5% for the respective metals. The adsorption process fitted satisfactorily with Freundlich isotherm with R2(0.999, 0.997, 0.995) for Cd2+, Pb2+, and Cr3+, respectively. The kinetic data obeyed the pseudo-second order for Cd2+ and Cr2+ and the pseudo-first order for Pb2+. Based on the results obtained within the framework of this study, it is concluded that the as-synthesized Co-DPA is a good adsorbent to eliminate heavy metal ions like Cd2+, Pb2+, and Cr3+from wastewater solution. In general, Co-DPA is a promising new material for the removal of heavy metal ions from water.

The receptor-bearing anthraquinone chromophore was synthesized by a simple aldamine condensation reaction, and its anion sensing properties were investigated via colorimetric, UV-vis, photoluminescence, and DFT calculations. The synthesized receptor detects both acetate and hypochlorite ions, where remarkable colorimetric transitions were observed from pink to purple for the acetate ion and pink to blue for the hypochlorite ion. Moreover, in the occurrence of the acetate ion, it shows an admirable answer for the Cr3+ ion, which changes its purple color to pink, while no notable change was observed for other ions. The detection limits of receptors with acetate and hypochlorite are 7.1 × 10-7 M and 9.4 × 10-7 M, respectively. The DFT calculation was performed to better understand the sensing mechanisms of both AcO- and ClO- ions. Furthermore, receptors were effectively utilized in the preparation of optical sensors supported by silica gel for the detection of AcO- and ClO- ions. The receptor proved itself to be potentially useful for real-life application by sensing AcO - in vinegar and ClO - ions in ala. Furthermore, its preeminent detection properties enabled the successful labeling of the AcO- ion in living biological cells.

Glutathione (GSH), an active peptide, plays pivotal roles in many physiological processes and detection of GSH inside of human body is of great importance for the playing of its biological effects. Here silver-phosphorus co-doped carbonized polymer dots (Ag@PCPDs) were prepared via solvothermal treatment of citric acid and phytic acid in the presence of Ag+ for GSH determination. The physicochemical and optical performance of the Ag@PCPDs were characterized by X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared (FT-IR), X-ray powder diffraction (XRD), Raman spectroscopy, transmission electron microscopy (TEM), fluorescence spectroscopy and ultraviolet-visible (UV-Vis) spectroscopy analyses. The prepared Ag@PCPDs have outstanding water solubility with high monodispersity (7.81 ± 0.31 nm) and exhibited excellent optical properties with excitation-dependent emission, high photostability, pH, and ionic strength tolerance. An optimized excitation at 358 nm, the Ag@PCPDs showed strong photoluminescent (PL) emission at 456 nm with a PL quantum yield (QYs) of 15.6%. Furthermore, the Ag@PCPDs were used as a PL sensing platform for detection GSH in a linear range of 0-200 μM with a low limit of detection at 0.68 μM. In addition, the proposed system can construct molecular logic gates with GSH and Fe3+ ions as the chemical inputs and PL emissions as the output. And the Ag@PCPDs were successfully used for GSH determination in real samples resulting in high sensitivity and satisfactory recoveries (92.81--107.45%). More importantly, the Ag@PCPDs showed low cytotoxicity at 500 μg/mL and superior cell imaging capability in HeLa cells, which offer a new path for detection and categorization of GSH in biomedical applications.