本试验旨在研究饲料中添加蝶芪(PTE)对ArborAcres肉鸡血清生化指标的影响。免疫和炎症反应,抗氧化状态,和肠道形态结构。持续42天,将480只1日龄的ArborAcres肉鸡随机分为四组。每组被分配接受基础饮食或补充200、400或600mg/kgPTE的基础饮食。每个处理由八个重复组成,每个复制品有15只小鸡。与对照组相比,3种PTE处理均能显著提高肉鸡脾脏淋巴细胞转化率。自动生化分析,酶联免疫吸附测定,和RT-qPCR分析试剂盒发现,400mg/kg的PTE显着增加了血清补体C3,IL-4和iNOS的水平;降低了血清IL-6,TNF-α,和基因IL-6,IL-8,TNF-α,NLRP3和IFN-γ;显著提高了包括CAT在内的抗氧化酶的活性,GSH-Px,和空肠中的T-SOD;并显着降低了肉仔鸡血清和空肠中的MDA含量。尼康显微镜观察和ImageProPlus6.0测量结果发现,400mg/kg的PTE补充剂显着降低了空肠的相对长度和重量,并改善了空肠绒毛结构,导致肠绒毛增加,加深的地穴,绒毛高度与隐窝深度之比(VH/CD)增强。RT-qPCR和Westernblot发现,饮食PTE还导致Claudin-2,Occludin,空肠中ZO-1和Sirt1和NF-κB蛋白水平降低。本研究结果表明,饲粮中添加PTE可通过减轻炎症反应和提高动物的抗氧化能力,改善肉鸡的免疫功能和肠道健康。
This experiment was carried out to investigate the effect of
pterostilbene (PTE) supplementation in feed on Arbor Acres broilers in terms of serum biochemical parameters, immune and inflammatory responses, antioxidant status, and intestinal morphological structure. For a duration of 42 days, a total of 480 1-day-old Arbor Acres broilers were randomly divided into four groups. Each group was assigned to receive either the basal diet or the basal diet supplemented with 200, 400, or 600 mg/kg of PTE. Each treatment consisted of eight replicates, with 15 chicks per replicate. In comparison with the control group, three PTE treatments significantly increased the lymphocyte transformation rate in the spleen of broilers. The automated biochemical analysis, enzyme-linked immunosorbent assay, and RT-qPCR analysis kits found that 400 mg/kg of PTE significantly increased the serum levels of complement C3, IL-4, and iNOS; reduced the serum levels of IL-6, TNF-α, and mRNA levels of the genes IL-6, IL-8, TNF-α, NLRP3, and IFN-γ; significantly improved the activities of antioxidant enzymes including CAT, GSH-Px, and T-SOD in the jejunum; and significantly reduced the MDA contents in the serum and jejunum of broilers. Nikon microscope observations and ImagePro Plus 6.0 measure results found that 400 mg/kg of PTE supplementation significantly reduced the relative length and weight of the jejunum and improved the jejunal villi structure, resulting in increased intestinal villi, deepened crypt, and an enhanced ratio of villi height to crypt depth (VH/CD). RT-qPCR and Western blot found that dietary PTE also resulted in increased mRNA levels of the genes Claudin-2, Occludin, ZO-1, and Sirt1, and decreased NF-κB protein levels in the jejunum. The results of this study demonstrated that dietary PTE improved the immune function and intestinal health of broilers by reducing inflammation and increasing the antioxidant capacity of the animals.