木薯(ManihotesculentaCrantz)块茎的采后快速生理退化(PPD)是储存过程中的重要问题。新鲜收获的块茎在24至72小时内开始变质。H2O2的积累是PPD期间发生的最早的生化事件之一,在两种对比木薯基因型中使用3,3二氨基联苯胺(DAB)检测到,MNP局部A(29-57μgg-1)和SreePrakash(64-141μgg-1)。在紫外线(UV)灯下观察到的木薯块茎发射的荧光羟基香豆素化合物的积累在储存的0、3、6、9、12和15天显示出显着变化。总酚和类胡萝卜素与PPD进展显著负相关;然而,花青素和类黄酮与PPD锚定的ROS积累呈正相关。主要化合物,邻苯二甲酸,二(2-丙基戊基)酯,在两个木薯块茎中都被发现,SreePrakash(57.21和35.21%),和MNP本地A(75.58和60.21%)在PPD的0和72小时,分别。PPD相关基因APX-2、APX-3、PAL、在PPD的6-12天时AP更高,这标志着活性氧周转的合成和苯丙素生物合成。一个重要的,坚强,次级代谢产物与PPD信号基因表达呈正相关,与羟基香豆素和H2O2的积累呈负相关。MNPLocalA块茎表现出15天的较长储存寿命,PPD评分较低,更高的代谢物合成,和基因表达。抗PPD品系可用于扩大木薯育种策略,用于大规模商业和工业用途。
Rapid postharvest physiological deterioration (PPD) in cassava (Manihot esculenta Crantz) tuber is a significant concern during storage. The freshly harvested tubers start spoiling within 24 to 72 h. Accumulation of H2O2 is one of the earliest biochemical events that occurred during PPD, which was detected using the 3,3 diaminobenzidine (DAB) in two contrast cassava genotypes, MNP Local A (29-57 μg g-1) and Sree Prakash (64-141 μg g-1). Accumulating the fluorescence hydroxycoumarin compounds emitted by the cassava tubers observed under an ultraviolet (UV) lamp showed significant variations at 0, 3, 6, 9, 12, and 15 days of storage. The total phenolics and carotenoids significantly and negatively correlated with PPD progression; however, the anthocyanin and flavonoids positively correlated with the PPD-anchored ROS accumulation. The primary compound, Phthalic acid, di(2-propylpentyl) ester, was identified in both the cassava tubers, Sree Prakash (57.21 and 35.21%), and MNP Local A (75.58 and 60.21%) at 0, and 72 h of PPD, respectively. The expression of PPD-associated genes APX-2, APX-3, PAL, and AP was higher at 6-12 days of PPD, which signified the synthesis of ROS turnover and phenylpropanoid biosynthesis. A significant, strong, and positive correlation was established between the secondary metabolites and PPD signaling gene expression, which was inversely correlated with hydroxycoumarin and H2O2 accumulation. MNP Local A tubers exhibited longer storage life of 15 days with a low PPD score, higher metabolites synthesis, and gene expression. The PPD-resistant lines may be used to augment cassava breeding strategies for large-scale commercial and industrial use.