Abstract：
Ethylene plays diverse roles in post-harvest processes of horticultural crops. However, its impact and regulation mechanism on the postharvest physiological deterioration (PPD) of cassava storage roots is unknown. In this study, a notable delay in PPD of cassava storage roots was observed when ethephon was utilized as an ethylene source. Physiological analyses and quantitative acetylproteomes were employed to investigate the regulation mechanism regulating cassava PPD under ethephon treatment. Ethephon was found to enhance the reactive oxygen species (ROS) scavenging system, resulting in a significant decrease in H2O2 and malondialdehyde (MDA) content. The comprehensive acetylome analysis identified 12,095 acetylation sites on 4403 proteins. Subsequent analysis demonstrated that ethephon can regulate the acetylation levels of antioxidant enzymes and members of the energy metabolism pathways. In summary, ethephon could enhance the antioxidant properties and regulate energy metabolism pathways, leading to the delayed PPD of cassava.
摘要：
乙烯在园艺作物的收获后过程中起着不同的作用。然而,其对木薯贮藏根采后生理退化(PPD)的影响及调控机制尚不清楚。在这项研究中,当乙烯利用作乙烯源时，木薯贮藏根的PPD显着延迟。采用生理分析和定量乙酰蛋白质组研究了乙烯利对木薯PPD的调控机制。发现乙烯利可增强活性氧（ROS）清除系统，导致H2O2和丙二醛（MDA）含量显着下降。全面的乙酰化分析确定了4403蛋白质上的12,095个乙酰化位点。随后的分析表明，乙烯利可调节抗氧化酶的乙酰化水平和能量代谢途径的成员。总之,乙烯利可以增强抗氧化性能，调节能量代谢途径，导致木薯PPD延迟。
