通常用作食品和化工行业的增塑剂,邻苯二甲酸二丁酯(DBP)在渗入或移动到周围环境中时对环境和人类福祉构成威胁。然而,关于DBP对水生生物危害的研究有限,其对干细胞和组织再生的影响尚未确定。Planarians,以其强大的再生能力和对水生污染物的敏感性而闻名,是毒理学中新兴的动物模型。本研究调查了环境相关水平的DBP对涡虫的综合毒性影响。它通过使用免疫荧光揭示了潜在的毒性机制,染色质分散测定,蛋白质印迹,定量实时荧光定量PCR(qRT-PCR),染色质行为和组织学分析,免疫荧光,和末端dUTP镍端标签(TUNEL)。研究结果表明,DBP引起形态和运动异常,组织损伤,再生抑制,和发育神经毒性。进一步的研究表明细胞凋亡增加,干细胞增殖和分化受到抑制,破坏细胞增殖和死亡的平衡,最终导致形态缺陷和功能异常。这归因于由活性氧(ROS)的过度释放引起的氧化应激和DNA损伤。这项探索为评估DBP对水生生物造成的毒性危险提供了新的视角。
Commonly utilized as a plasticizer in the food and chemical sectors, Dibutyl phthalate (DBP) poses threats to the environment and human well-being as it seeps or moves into the surroundings. Nevertheless, research on the harmfulness of DBP to aquatic organisms is limited, and its impact on stem cells and tissue regeneration remains unidentified.
Planarians, recognized for their robust regenerative capabilities and sensitivity to aquatic pollutants, are emerging animal models in toxicology. This study investigated the comprehensive toxicity effects of environmentally relevant levels of DBP on
planarians. It revealed potential toxicity mechanisms through the use of immunofluorescence, chromatin dispersion assay, Western blot, quantitative real-time fluorescence quantitative PCR (qRT-PCR), chromatin behavioral and histological analyses, immunofluorescence, and terminal dUTP nickel-end labeling (TUNEL). Findings illustrated that DBP caused morphological and motor abnormalities, tissue damage, regenerative inhibition, and developmental neurotoxicity. Further research revealed increased apoptosis and suppressed stem cell proliferation and differentiation, disrupting a balance of cell proliferation and death, ultimately leading to morphological defects and functional abnormalities. This was attributed to oxidative stress and DNA damage caused by excessive release of reactive oxygen species (ROS). This exploration furnishes fresh perspectives on evaluating the toxicity peril posed by DBP in aquatic organisms.