Abstract：
Using immunocytochemistry, serotonergic nerve elements were documented in the nervous system of the planarian Girardia tigrina. Serotonin-immunopositive components were observed in the brain, ventral, dorsal and longitudinal nerve cords, transverse nerve commissures connecting the nerve cords, and in the nerve plexus. Whole-mount preparations of G. tigrina were analyzed by fluorescent and confocal laser scanning microscopy. An essential quantitative morphometric measurement of serotonin-immunopositive structures was conducted in three body regions (anterior, middle, and posterior) of the planarian. The number of serotonin neurons was maximal in the head region. The ventral nerve cords gradually decreased in thickness from anterior to posterior body ends. Physiological action of exogenously applied serotonin was studied in G. tigrina for the first time. It was found that serotonin (0.1 and 1 µmol L-1) accelerated eye regeneration. The transcriptome sequencing performed for the first time for the planarian G. tigrina revealed the transcripts of the tryptophan hydroxylase (trph), amino acid decarboxylase (aadc) and serotonin transporter (sert) genes. The data obtained indicate the presence of the components of serotonin pathway in G. tigrina. The identified transcripts can take part in serotonin turnover and participate in the realization of biological effects of serotonin in planarians, associated with eyes regeneration and differentiation.
摘要：
使用免疫细胞化学，在涡虫Girardiatigrina的神经系统中记录了5-羟色胺能神经元素。在大脑中观察到血清素免疫阳性成分，腹侧,背侧和纵向神经索，连接神经索的横神经连合，在神经丛中。通过荧光和共聚焦激光扫描显微镜分析了G.tigrina的整装制剂。在三个身体区域（前，中间,和涡虫的后部）。头部区域的5-羟色胺神经元数量最大。腹侧神经索的厚度从身体的前端到后端逐渐减少。首次在G.tigrina中研究了外源施用5-羟色胺的生理作用。发现血清素（0.1和1µmolL-1）加速了眼睛的再生。首次对涡虫G.tigrina进行的转录组测序揭示了色氨酸羟化酶（trph）的转录本，氨基酸脱羧酶（aadc）和5-羟色胺转运蛋白（sert）基因。所获得的数据表明在G.tigrina中存在5-羟色胺途径的组分。鉴定的转录本可以参与5-羟色胺的周转，参与5-羟色胺生物学效应的实现。与眼睛的再生和分化有关。
