术后粘连,手术的常见并发症,引起疼痛,损害器官功能,通常需要额外的手术干预。控制炎症,保护受伤的组织,和快速组织修复是预防粘连的关键。粘附屏障是用于通过相对的损伤组织的物理分离来防止粘连的生物材料。目前的粘连屏障具有较差的抗炎和组织再生特性。脐带组织(UT),胎盘的一部分,本质上是柔软的,符合,生物相容性和可生物降解的,抗菌药物,抗炎,和抗纤维化特性,使其成为目前可用的粘合屏障的有吸引力的替代品。虽然使用新鲜的组织是优选的,可用性和较短的储存时间限制了其临床使用。一种可行的冷冻保存的UT(vCUT)“护理点”同种异体移植物最近已成为可用。vCUT保留细胞外基质,生长因子,和天然活细胞,具有在-80°C下的长保质期的额外优点。在这项研究中,在兔腹部粘连模型中评估vCUT的抗粘连性能。盲肠在相反的两侧被磨损,将vCUT缝合到治疗侧的腹壁;而腹部的对侧用作内部未治疗的对照。在手术后7、28和67天进行总体和组织学评估。在所有时间点,在vCUT处理侧上均未检测到粘连。粘连的组织学评分,炎症,与对照侧相比,vCUT治疗侧的纤维化较低。总之,数据支持在外科手术中使用vCUT作为粘连屏障.
Post-operative adhesions, a common complication of surgery, cause pain, impair organ functionality, and often require additional surgical interventions. Control of inflammation, protection of injured tissue, and rapid tissue repair are critical for adhesion prevention. Adhesion barriers are biomaterials used to prevent adhesions by physical separation of opposing injured tissues. Current adhesion barriers have poor anti-inflammatory and tissue regenerative properties. Umbilical cord tissue (UT), a part of the placenta, is inherently soft, conforming, biocompatible, and biodegradable, with antimicrobial, anti-inflammatory, and antifibrotic properties, making it an attractive alternative to currently available adhesion barriers. While use of fresh tissue is preferable, availability and short storage time limit its clinical use. A viable cryopreserved UT (vCUT) \"point of care\" allograft has recently become available. vCUT retains the extracellular matrix, growth factors, and native viable cells with the added advantage of a long shelf life at -80 °C. In this study, vCUT\'s anti-adhesion property was evaluated in a rabbit abdominal adhesion model. The cecum was abraded on two opposing sides, and vCUT was sutured to the abdominal wall on the treatment side; whereas the contralateral side of the abdomen served as an internal untreated control. Gross and histological evaluation was performed at 7, 28, and 67 days post-surgery. No adhesions were detectable on the vCUT treated side at all time points. Histological scores for adhesion, inflammation, and fibrosis were lower on the vCUT treated side as compared to the control side. In conclusion, the data supports the use of vCUT as an adhesion barrier in surgical procedures.