进行了两个实验来评估以500植酸酶单位(FTU)/kg日粮添加的生物合成6-植酸酶对生长性能的影响,骨矿化,断奶仔猪和生长肥育猪的养分消化率和保留率。对90头断奶的雄性和雌性仔猪进行了实验,平均初始体重(BW)为7.7±0.73kg,26日龄)和300只雄性和雌性生长猪(初始BW:21.0±3.44kg),分别在实验1和2中持续43和98天。在每个实验中,根据随机完全区组设计,将动物分配到三种治疗中的一种.治疗包括为满足营养需求而配制的阳性对照(PC)饮食;在第1阶段和第2阶段,阴性对照(NC)饮食中的钙(Ca)和可消化P分别降低了0.15%和0.12%,仔猪分别为0.14、0.11和0.10%点,分别,在生长肥育猪的第一、第二和第三阶段,与PC饮食相比;以及以500FTU/kg饮食(PHY)补充新的6-植酸酶的NC饮食。饮食中的磷和钙消耗降低了(p<0.05)最终BW(-11.9%;-7.8%,),平均每日收益(ADG,-17.8%;-10.1%),平均日采食量(ADFI,-9.9%;-6.0%),增料比(G:F)(-8.9%;-4.6%),和P的表观总道消化率(ATTD)(-7.7%点;-6.7%点)在苗圃仔猪和生长猪,分别。它还降低了(p<0.001)P和Ca保留6.1和9.4%点,分别,在保育猪和灰烬中,P,掌骨中的Ca含量分别为18.4、18.4和16.8%,分别,在成长的猪与饲喂NC饮食的动物相比,植酸酶补充提高了(p<0.001)最终BW(+7.7%;+11.3%),ADG(+12.5%;+15.0%),G:F比值(+8.4%;+5.8%),Ca的ATTD(+10.8%点;+7.2%点),断奶仔猪和生长猪的P的ATTD(+18.7%点;+16.6%点),分别。此外,植酸酶还增加了(p<0.001)6.1%和9.4%的P和Ca保留,分别,在保育猪和灰烬中,P,掌骨中的Ca含量分别为17.7、15.0和15.2%,分别,在成长的猪最后的BW,ADG,G:F比值,饲喂补充植酸酶的NC饮食的动物的骨特征与饲喂PC饮食的动物相当。这一发现表明,这种新型生物合成植酸酶能够通过改善仔猪和饲喂缺乏P和Ca的饮食的生长猪中P和Ca的利用率来恢复性能和骨矿化。
Two experiments were performed to evaluate the effect of a biosynthetic 6-
phytase added at 500
phytase unit (FTU)/kg diet on growth performance, bone mineralization, and nutrient digestibility and retention in weaned piglets and growing-finishing pigs. Experiments were performed on 90 weaned male and female piglets with an average initial body weight (BW) at 7.7 ± 0.73 kg, 26 days of age) and 300 male and female growing pigs (initial BW: 21.0 ± 3.44 kg) for 43 and 98 days in experiments 1 and 2, respectively. In each experiment, the animals were assigned to one of three treatments according to a randomized complete block design. The treatments consisted of a positive-control (PC) diet formulated to meet nutrient requirements; a negative-control (NC) diet reduced similarly in calcium (Ca) and digestible P by 0.15 and 0.12% points in phases 1 and 2, respectively, in piglets and by 0.14, 0.11, and 0.10% points, respectively, in phases 1, 2, and 3 in growing-finishing pigs, compared with PC diet; and a NC diet supplemented with the new 6-phytase at 500 FTU/kg diet (PHY). The dietary P and Ca depletion reduced (p < 0.05) the final BW (-11.9%; -7.8%,), average daily gain (ADG, -17.8%; -10.1%), average daily feed intake (ADFI, -9.9%; -6.0%), gain-to-feed (G:F) ratio (-8.9%; -4.6%), and apparent total tract digestibility (ATTD) of P (-7.7% points; -6.7% points) in nursery piglets and growing pigs, respectively. It also decreased (p < 0.001) P and Ca retention by 6.1 and 9.4% points, respectively, in nursery pigs and ash, P, and Ca contents in metacarpal bones by 18.4, 18.4, and 16.8%, respectively, in growing pigs. Compared to animals fed the NC diet,
phytase supplementation improved (p < 0.001) the final BW (+7.7%; +11.3%), ADG (+12.5%; +15.0%), G:F ratio (+8.4%; +5.8%), ATTD of Ca (+10.8% points; +7.2% points), and ATTD of P (+18.7% points; +16.6% points) in weaned piglets and growing pigs, respectively. In addition,
phytase also increased (p < 0.001) P and Ca retention by 6.1 and 9.4% points, respectively, in nursery pigs and ash, P, and Ca contents in metacarpal bones by 17.7, 15.0, and 15.2%, respectively, in growing pigs. The final BW, ADG, G:F ratio, and bone traits in animals fed the NC diet supplemented with
phytase were comparable to animals fed the PC diet. This finding indicates the ability of this novel biosynthetic
phytase to restore performance and bone mineralization by improving the availability of P and Ca in piglets and growing pigs fed P- and Ca-deficient diets.