PDF(Pubmed)
Abstract：
Phosphorus (P) is essential for biological systems, playing a pivotal role in energy metabolism and forming crucial structural components of DNA and RNA. Yet its bioavailable forms are scarce. Phytate, a major form of stored phosphorus in cereals and soils, is poorly bioavailable due to its complex structure. Phytases, enzymes that hydrolyze phytate to release useable phosphorus, are vital in overcoming this limitation and have significant biotechnological applications. This study employed novel method to isolate and characterize bacterial strains capable of metabolizing phytate as the sole carbon and phosphorus source from the Andes mountains soils. Ten strains from the genera Klebsiella and Chryseobacterium were isolated, with Chryseobacterium sp. CP-77 and Klebsiella pneumoniae CP-84 showing specific activities of 3.5 ± 0.4 nkat/mg and 40.8 ± 5 nkat/mg, respectively. Genomic sequencing revealed significant genetic diversity, suggesting CP-77 may represent a novel Chryseobacterium species. A fosmid library screening identified several phytase genes, including a 3-phytase in CP-77 and a glucose 1-phosphatase and 3-phytase in CP-84. Phylogenetic analysis confirmed the novelty of these enzymes. These findings highlight the potential of phytase-producing bacteria in sustainable agriculture by enhancing phosphorus bioavailability, reducing reliance on synthetic fertilizers, and contributing to environmental management. This study expands our biotechnological toolkit for microbial phosphorus management and underscores the importance of exploring poorly characterized environments for novel microbial functions. The integration of direct cultivation with metagenomic screening offers robust approaches for discovering microbial biocatalysts, promoting sustainable agricultural practices, and advancing environmental conservation.
摘要：
磷（P）对生物系统至关重要，在能量代谢中起关键作用，并形成DNA和RNA的关键结构成分。然而，它的生物可利用形式很少。植酸盐,谷物和土壤中储存的磷的主要形式，由于其复杂的结构，生物可利用性差。植酸酶,水解植酸盐以释放可用磷的酶，对于克服这一限制至关重要，并具有重要的生物技术应用。这项研究采用了新颖的方法来分离和表征能够代谢植酸作为安第斯山脉土壤中唯一碳和磷源的细菌菌株。从克雷伯菌属和金黄杆菌属中分离出10株菌株，与金杆菌属。CP-77和肺炎克雷伯菌CP-84显示3.5±0.4nkat/mg和40.8±5nkat/mg的比活性，分别。基因组测序揭示了显著的遗传多样性，表明CP-77可能代表了一种新的金黄杆菌属物种。Fosmid文库筛选确定了几个植酸酶基因，包括CP-77中的3-植酸酶和CP-84中的葡萄糖1-磷酸酶和3-植酸酶。系统发育分析证实了这些酶的新颖性。这些发现强调了通过提高磷的生物利用度，产生植酸酶的细菌在可持续农业中的潜力，减少对合成肥料的依赖，为环境管理做出贡献。这项研究扩展了我们用于微生物磷管理的生物技术工具包，并强调了探索特征不佳的环境以实现新型微生物功能的重要性。直接培养与宏基因组筛选的整合为发现微生物生物催化剂提供了强大的方法，促进可持续农业实践，推进环境保护。
