耐药大肠杆菌(E.由于过度使用抗生素而导致的大肠杆菌)需要立即探索替代方法来抵消致病性大肠杆菌。噬菌体,凭借其独特的抗菌机制,被认为是治疗细菌感染的有希望的候选人。在这里,我们分离了一种裂解性大肠杆菌噬菌体TequatrovirusYZ2(噬菌体YZ2),属于Tequatrovirus属。噬菌体YZ2的基因组由168,356个碱基对组成,GC含量为35.34%和269个推定的开放阅读框(ORF)。其中,146个ORF已被注释为与核苷酸代谢相关的功能蛋白,结构,转录,DNA复制,翻译,和裂解。在大肠杆菌感染的皮肤伤口的小鼠模型中,噬菌体YZ2治疗显著促进伤口愈合。此外,组织病理学分析显示IL-1β和TNF-α水平降低,VEGF水平升高,表明噬菌体作为抗大肠杆菌感染的有效抗微生物剂的潜力。
The increasing prevalence of drug-resistant Escherichia coli (E. coli) resulting from the excessive utilization of antibiotics necessitates the immediate exploration of alternative approaches to counteract pathogenic E. coli. Phages, with their unique antibacterial mechanisms, are considered promising candidates for treating bacterial infections. Herein, we isolated a lytic Escherichia phage Tequatrovirus YZ2 (phage YZ2), which belongs to the genus Tequatrovirus. The genome of phage YZ2 consists of 168,356 base pairs with a G + C content of 35.34% and 269 putative open reading frames (ORFs). Of these, 146 ORFs have been annotated as functional proteins associated with nucleotide metabolism, structure, transcription, DNA replication, translation, and lysis. In the mouse model of a skin wound infected by E. coli, phage YZ2 therapy significantly promoted the wound healing. Furthermore, histopathological analysis revealed reductions in IL-1β and TNF-α and increased VEGF levels, indicating the potential of phages as effective antimicrobial agents against E. coli infection.