沙门氏菌多重耐药性的出现,引起食源性感染,是一个重要的问题。在沙门氏菌中拥有超过2,600个血清型。,确定每个血清型的具体解决方案至关重要。噬菌体疗法作为替代治疗选择。在这项研究中,从污水中获得vB_SalP_792噬菌体,在13个测试的临床肠链球菌分离株中的8个中形成斑块。透射电子显微镜(TEM)检查显示T7样形态。噬菌体的特点是它的稳定性,生命周期,抗生物膜,和食物来源的裂解能力。噬菌体在整个温度范围内(-20至70°C)保持稳定,pH值(3-11),在氯仿和乙醚中。它还在0.0001至100的MOI范围内表现出裂解活性。生命周期显示,95%的噬菌体在3分钟内附着在其宿主上,接下来是5分钟的潜伏期,导致50PFU/单元的突发大小。vB_SalP_792噬菌体基因组具有长度为37,281bp和GC含量为51%的dsDNA。有42个编码序列(CDS),24个具有推定功能,没有抗性或毒力相关基因。vB_SalP_792噬菌体显着降低了已建立的生物膜以及蛋白中的细菌负荷。因此,vB_SalP_792噬菌体可以作为一种有效的生物防治剂,用于预防食物中的沙门氏菌感染,以及它对肠球菌临床分离株的有效裂解活性,列出了vB_SalP_792噬菌体作为未来针对耐药沙门氏菌感染的体内研究和治疗应用的成功候选者。
The emergence of multi-drug resistance in Salmonella, causing food-borne infections, is a significant issue. With over 2,600 serovars in in Salmonella sp., it is crucial to identify specific solutions for each serovar. Phage therapy serves as an alternate treatment option. In this study, vB_SalP_792 phage was obtained from sewage, forming plaques in eight out of 13 tested clinical S. enterica isolates. Transmission electron microscopy (TEM) examination revealed a T7-like morphotype. The phage was characterized by its stability, life cycle, antibiofilm, and lytic ability in food sources. The phage remains stable throughout a range of temperatures (-20 to 70°C), pH levels (3-11), and in chloroform and ether. It also exhibited lytic activity within a range of MOIs from 0.0001 to 100. The life cycle revealed that 95% of the phages attached to their host within 3 min, followed by a 5-min latent period, resulting in a 50 PFU/cell burst size. The vB_SalP_792 phage genome has a dsDNA with a length of 37,281 bp and a GC content of 51%. There are 42 coding sequences (CDS), with 24 having putative functions and no resistance or virulence-related genes. The vB_SalP_792 phage significantly reduced the bacterial load in the established biofilms and also in egg whites. Thus, vB_SalP_792 phage can serve as an effective biocontrol agent for preventing Salmonella infections in food, and its potent lytic activity against the clinical isolates of S. enterica, sets out vB_SalP_792 phage as a successful candidate for future in vivo studies and therapeutical application against drug-resistant Salmonella infections.