背景:侧流免疫测定(LFIA)被广泛用作即时检测(POCT)技术。然而,其有限的灵敏度阻碍了其在低丰度生物标志物检测中的应用。最近,已经实施了纳米酶的利用,通过催化3,3\'的氧化来增强LFIA的敏感性,5,5'-四甲基联苯胺(TMB)。纳米酶的催化性能在影响LFIA的敏感性中起着至关重要的作用。
结果:山茱萸。具有良好过氧化物酶样活性的等Zucc-Pd@Pt(CO-Pd@Pt)纳米酶在本文中通过使用山茱萸Sieb的简易一锅法合成。等Zucc提取物作为还原剂。用TEM表征了CO-Pd@Pt纳米酶的形态和组成,SEM,XRD,和XPS。作为概念的证明,合成的CO-Pd@Pt纳米酶用于LFIA(CO-Pd@Pt-LFIA)检测人绒毛膜促性腺激素(hCG)。与传统的基于金纳米粒子的LFIA(AuNPs-LFIA)相比,CO-Pd@Pt-LFIA显示检测限的显着提高(LOD,0.08mIU/mL),比AuNP-LFIA低约160倍。此外,实验评估准确性,精度,选择性,干扰,和稳固性证实了CO-Pd@Pt-LFIA用于hCG含量测定的实际适用性。
结论:本研究提出了一种通过环境友好方法合成双金属纳米酶的新方法,利用植物提取物作为保护剂和还原剂。此外,提出了一种易于实现的技术来增强侧流免疫分析中的信号检测。
BACKGROUND: The lateral flow immunoassay (LFIA) is widely employed as a point-of-care testing (POCT) technique. However, its limited sensitivity hinders its application in detecting biomarkers with low abundance. Recently, the utilization of nanozymes has been implemented to enhance the sensitivity of LFIA by catalyzing the oxidation of 3,3\',5,5\'-tetramethylbenzidine (TMB). The catalytic performance of nanozymes plays a crucial role in influencing the sensitivity of LFIA.
RESULTS: The Cornus officinalis Sieb. et Zucc-Pd@Pt (CO-Pd@Pt) nanozyme with good peroxidase-like activity was synthesized herein through a facile one-pot method employing Cornus officinalis Sieb. et Zucc extract as a reducing agent. The morphology and composition of the CO-Pd@Pt nanozyme were characterized using TEM, SEM, XRD, and XPS. As a proof of concept, the as-synthesized CO-Pd@Pt nanozyme was utilized in LFIA (CO-Pd@Pt-LFIA) for the detection of human chorionic gonadotropin (hCG). Compared to conventional gold nanoparticles-based LFIA (AuNPs-LFIA), CO-Pd@Pt-LFIA demonstrated a significant enhancement in the limit of detection (LOD, 0.08 mIU/mL), which is approximately 160 times lower than that of AuNPs-LFIA. Furthermore, experiments evaluating accuracy, precision, selectivity, interference, and stability have confirmed the practical applicability of CO-Pd@Pt-LFIA for hCG content determination.
CONCLUSIONS: The present study presents a novel approach for the synthesis of bimetallic nanozymes through environmentally friendly methods, utilizing plant extracts as both protective and reducing agents. Additionally, an easily implementable technique is proposed to enhance signal detection in lateral flow immunoassays.