Abstract：
In this work, the B, N co-doped carbon dots (B, N-CDs) were synthesized via facile hydrothermal approach with 6-aminopyridine boronic acid as precursor. In addition to emitting intense blue luminescence when exposed to ultraviolet light, the prepared B, N-CDs displayed remarkable peroxidase-like activity, which could efficiently catalyze the oxidation of 3, 3\', 5, 5\' -tetramethylbenzidine (TMB) to blue ox-TMB in the presence of hydrogen peroxide (H2O2). Furthermore, the fluorescence intensity of B, N-CDs increased gradually upon the addition of H2O2. Since cholesterol oxidase (ChOx) can catalyze the oxidation of cholesterol to form H2O2, the as-prepared B, N-CDs was then used as both colorimetric and fluorometric sensors for the detection of cholesterol with detection limit of 0.87 and 2.31 μM, respectively. Finally, the dual-mode approach based on B, N-CDs was effectively utilized for detecting cholesterol levels in serum samples, proving the potential application of B, N-CDs in the field of biological assay.
摘要：
在这项工作中,B、N共掺杂碳点（B，以6-氨基吡啶硼酸为前体，通过简单的水热法合成了N-CD）。除了在暴露于紫外光时发出强烈的蓝色发光，准备好的B，N-CD表现出明显的过氧化物酶样活性，可以有效催化3，3'的氧化，在过氧化氢(H2O2)存在下,5,5'-四甲基联苯胺(TMB)至蓝色ox-TMB。此外，B的荧光强度，添加H2O2后，N-CD逐渐增加。由于胆固醇氧化酶(ChOx)能催化胆固醇氧化生成H2O2,然后将N-CD用作比色和荧光传感器，用于检测胆固醇，检出限为0.87和2.31μM，分别。最后,基于B的双模方法，N-CD被有效地用于检测血清样本中的胆固醇水平，证明了B的潜在应用，生物测定领域中的N-CD。
