PDF(Pubmed)
Abstract：
Co-N-CDs-based MXene nanocomposites (MXene@PDA/Co-N-CDs) were constructed by decorating Co-N-CDs on polydopamine-functionalized MXene nanosheets. Both Co-N-CDs and MXene nanosheets have peroxidase-like activity; when the two materials are combined to form MXene@PDA/Co-N-CDs nanocomposites, the peroxide-like activity can be further enhanced. MXene@PDA/Co-N-CDs could oxidize the substrate 3,3\'5,5\'-tetramethylbenziline (TMB) to form ox-TMB, as confirmed by detecting the absorption of the blue products. A highly selective colorimetric biosensor was developed for the determination of glutathione (GSH) in the concentration range of 0.3 to 20 µM with a lower detection limit (LOD) of 0.12 µM, which realized the accurate detection of GSH in human serum and urine samples. Moreover, in the tumor microenvironment, MXene@PDA/Co-N-CDs could catalyze hydrogen peroxide to produce hydroxyl free radicals and produce a photothermal effect under the exposure of NIR-I irradiation. The catalytic activity of MXene@PDA/Co-N-CD nanocomposites was fully achieved for the death of cancer cells through photothermal/photodynamic synergistic therapy. The MXene@PDA/Co-N-CDs nanozyme offers multiple applications in GSH detection and tumor therapy.
摘要：
通过在聚多巴胺功能化的MXene纳米片上装饰Co-N-CD来构建基于Co-N-CD的MXene纳米复合材料（MXene@PDA/Co-N-CD）。Co-N-CD和MXene纳米片都具有过氧化物酶样活性；当两种材料结合形成MXene@PDA/Co-N-CD纳米复合材料时，过氧化物样活性可以进一步增强。MXene@PDA/Co-N-CD可以氧化底物3,3\'5,5'-四甲基苯胺（TMB）形成ox-TMB，通过检测蓝色产品的吸收证实。开发了一种高选择性比色生物传感器，用于测定浓度范围为0.3至20µM的谷胱甘肽（GSH），检测下限（LOD）为0.12µM，实现了人血清和尿液中GSH的准确检测。此外，在肿瘤微环境中，MXene@PDA/Co-N-CD可以催化过氧化氢产生羟基自由基，并在NIR-I辐照下产生光热效应。MXene@PDA/Co-N-CD纳米复合材料通过光热/光动力协同治疗完全实现了对癌细胞死亡的催化活性。MXene@PDA/Co-N-CD纳米酶在GSH检测和肿瘤治疗中提供多种应用。
