在成年小鼠的延髓脊髓中央管(CC)周围发现了与脑脊液(CSF)接触的神经元。这些神经元(CSF-CN),位于室管膜细胞层内或下方,被称为干细胞生态位,呈现特征形态,枝晶突出到CC并以突起结束。它们是GABA能的,呈现中等神经元成熟度并选择性表达PKD2L1,这是具有感觉特性的瞬时受体电位通道超家族的成员。在小鼠中使用免疫组织学和电生理记录技术,我们表征了远离CC的新PKD2L1阳性细胞群的特性,该细胞位于富含星形胶质细胞和腹中脊髓和髓质室管膜纤维的区域。它们出现在胚胎第16天左右,并且它们的数量增加到产后早期。随着CC地区的发展和重组,它们越来越远离CC,暗示了一些迁徙能力。这些神经元与CSF-cN共享功能和表型特性,但似乎分为两组。一组,出现在中线,具有双极形态,并沿着室管膜纤维并向CC延伸长枝晶。第二组,位于更多的腹侧区域,具有多极形态,对CC没有明显的投影。总之,我们描述了远离CC的PKD2L1+神经元的新群体,但具有与CSF-cNs相似的特性,可能有助于对CSF或间质液的组成进行有义修饰。需要确认的功能。
Neurons in contact with the cerebrospinal fluid (CSF) are found around the medullo-spinal central canal (CC) in adult mice. These neurons (CSF-cNs), located within or below the ependymal cell layer, known as the stem cell niche, present a characteristic morphology with a dendrite projecting to the CC and ending with a protrusion. They are GABAergic, present an intermediate neuronal maturity and selectively express
PKD2L1, a member of the transient receptor potential channel superfamily with sensory properties. Using immunohistological and electrophysiological recording techniques in mice, we characterize the properties of a new population of
PKD2L1 positive cells that is distant from the CC in a zone enriched with astrocytes and ependymal fibers of the ventro-medial spinal cord and medulla. They appear around embryonic day 16 and their number increases up to early postnatal days. With development and the reorganization of the CC region, they progressively become more distant from the CC, suggesting some migratory capabilities. These neurons share functional and phenotypical properties with CSF-cNs but appear subdivided in two groups. One group, present along the midline, has a bipolar morphology and extends a long dendrite along ependymal fibers and towards the CC. The second group, localized in more ventro-lateral regions, has a multipolar morphology and no apparent projection to the CC. Altogether, we describe a novel population of
PKD2L1+ neurons distant from the CC but with properties similar to CSF-cNs that might serve to sense modification in the composition of either CSF or interstitial liquid, a function that will need to be confirmed.