敌敌畏是一种有机磷农药,通常用于农业和家庭控制害虫和昆虫。尽管它有用,它具有生殖毒性并诱发男性性功能障碍。另一方面,据报道,姜黄素可改善性功能障碍。然而,直到日期,尚无研究报告姜黄素对敌敌畏引起的性功能障碍的影响.本研究探讨姜黄素对敌敌畏性性功能障碍的影响及相关机制。将32只雄性Wistar大鼠随机分为四组;对照组(1毫升橄榄油),姜黄素治疗(100mg/kg),DDVP处理(98.54g/m3敌敌畏吸入),和DDVP+姜黄素治疗。敌敌畏诱发的性功能障碍,表现为交配动机降低(8.38±0.18vs.4.00±0.33,P<0.0001),延长潜伏期(46.63±1.30vs.98.75±1.32,P<0.0001)和降低的安装频率(14.88±0.52vs.8.63±0.38),内渗(9.38±0.50vs.3.75±0.31,P<0.0001),和射精(7.63±0.38vs.1.50±0.19,P<0.0001)。这些发现伴随着下丘脑-垂体-睾丸轴的抑制,循环FSH显着减少(60.00±1.04vs.21.13±0.52,P<0.0001),LH(46.38±1.38vs.19.00±0.46,P<0.0001),和睾丸激素(6.01±0.50vs.0.74±0.05,P<0.0001)。尽管如此,在敌敌畏暴露的大鼠中施用姜黄素通过改善雄性性行为的评估指标,可显着减轻敌敌畏引起的性功能障碍。此外,姜黄素显着增加FSH的血清水平(21.13±0.52vs.47.25±0.10,P<0.0001),LH(19.00±0.46vs.43.00±1.49),和睾酮(0.74±0.05vs.3.98±0.08,P<0.0001)。这项研究表明,姜黄素通过激活下丘脑-垂体-睾丸轴和上调循环睾丸激素来减轻敌敌畏引起的性功能障碍。
Dichlorvos is an
organophosphate pesticide that is commonly used for agricultural and domestic control of pests and insects. Despite its usefulness, it exerts reproductive toxicity and induces male sexual dysfunction. On the other hand, curcumin has been reported to improve sexual dysfunction. However, till date, no study has reported the impact of curcumin on dichlorvos-induced sexual dysfunction. This study investigated the effect and associated mechanism of curcumin on dichlorvos-induced sexual dysfunction. Thirty-two male Wistar rats were randomized into four groups; the control (1 mL of olive oil), curcumin-treated (100 mg/kg), DDVP-treated (98.54 g/m3 of dichlorvos by inhalation), and DDVP + Curcumin-treated. Dichlorvos induced sexual dysfunction as depicted by reduced motivation to mate (8.38 ± 0.18 vs. 4.00 ± 0.33, P < 0.0001), prolonged latencies (46.63 ± 1.30 vs. 98.75 ± 1.32, P < 0.0001) and reduced frequencies of mount (14.88 ± 0.52 vs. 8.63 ± 0.38), intromission (9.38 ± 0.50 vs. 3.75 ± 0.31, P < 0.0001), and ejaculation (7.63 ± 0.38 vs. 1.50 ± 0.19, P < 0.0001). These findings were accompanied by suppression of hypothalamic-pituitary-testicular axis, evidenced by marked reductions in circulating FSH (60.00 ± 1.04 vs. 21.13 ± 0.52, P < 0.0001), LH (46.38 ± 1.38 vs. 19.00 ± 0.46, P < 0.0001), and testosterone (6.01 ± 0.50 vs. 0.74 ± 0.05, P < 0.0001). Nonetheless, the administration of curcumin in dichlorvos-exposed rats significantly attenuated dichlorvos-induced sexual dysfunction by improving the assessed indices of male sexual act. Also, curcumin significantly increased serum levels of FSH (21.13 ± 0.52 vs. 47.25 ± 0.10, P < 0.0001), LH (19.00 ± 0.46 vs. 43.00 ± 1.49), and testosterone (0.74 ± 0.05 vs. 3.98 ± 0.08, P < 0.0001). This study revealed that curcumin attenuated dichlorvos-induced sexual dysfunction by activating the hypothalamic-pituitary-testicular axis and upregulating circulating testosterone.