麦冬,中文也叫麦东,主要在四川和浙江两省生产:“川麦洞(CMD)”和“哲麦洞(ZMD)”,\"分别。本研究旨在区分和评价CMD和ZMD的质量。在这项研究中,使用UPLC-Q/TOF-MS研究了CMD和ZMD的块茎,GC-MS,和LC-MS方法,分别。总的来说,甾体皂苷,高异黄酮,氨基酸,核苷很快就被鉴定出来了。此外,多元统计分析显示CMD和ZMD可以分离。此外,CMD显示较高水平的4-氨基丁酸,甘氨酸,l-脯氨酸,单乙醇胺,和丝氨酸比ZMD。此外,绿原酸的含量,创伤性酸,胞苷,尸体,吡哆醇5-磷酸,谷蛋白,ZMD和天花苷3-O-(6-O-丙二酰-β-d-葡萄糖苷)明显高于CMD。此外,这些不同的成分主要与半乳糖代谢;淀粉和蔗糖代谢;半胱氨酸和蛋氨酸代谢;缬氨酸,亮氨酸,和异亮氨酸生物合成;和甘油磷脂代谢。总的来说,这些结果表明,不同产地麦冬的生物活性化学成分之间存在许多差异,其中ZMD在质量评估方面比CMD表现更好,和UPLC-Q/TOF-MS,GC-MS,LC-MS是鉴别不同产地药材的有效方法。
Ophiopogonis Radix, also known as Maidong in Chinese, is largely produced in the Sichuan and Zhejiang provinces: \"Chuan-maidong (CMD)\" and \"Zhe-maidong (ZMD),\" respectively. This study aimed to distinguish and evaluate the quality of CMD and ZMD. In this study, the tubers of CMD and ZMD were investigated using UPLC-Q/TOF-MS, GC-MS, and LC-MS methods, respectively. Overall, steroidal saponins, homoisoflavonoids, amino acids, and nucleosides were quickly identified. Furthermore, multivariate statistical analysis revealed that CMD and ZMD could be separated. Moreover, CMD showed higher levels of 4-aminobutanoic acid, glycine, l-proline, monoethanolamine, and serine than ZMD. Besides, the levels of chlorogenic acid, traumatic acid, cytidine, cadaverine, pyridoxine 5-phosphate, glutinone, and pelargonidin 3-O-(6-O-malonyl-β-d-glucoside) were remarkably higher in ZMD than in CMD. Furthermore, these different constituents were mainly associated with galactose metabolism; starch and sucrose metabolism; cysteine and methionine metabolism; valine, leucine, and isoleucine biosynthesis; and glycerophospholipid metabolism. In general, these results showed many differences between the bioactive chemical constituents of Ophiopogon japonicus from different production areas, where ZMD performed better in the quality assessment than CMD, and that UPLC-Q/TOF-MS, GC-MS, and LC-MS are effective methods to discriminate medicinal herbs from different production areas.