■过敏性鼻炎(AR)是由鼻子中的IgE介导的I型超敏反应。硫氧还蛋白相互作用蛋白(TXNIP)在活性氧(ROS)的产生过程中起着关键作用。白藜芦醇是TXNIP抑制剂。尽管如此,其在AR中的作用和机制尚不明确。本研究旨在探讨白藜芦醇对卵清蛋白(OVA)诱导的AR小鼠模型的作用及机制。
■使用OVA建立AR鼠模型并鼻内施用白藜芦醇或N-乙酰半胱氨酸(NAC)。苏木精和伊红(HE)染色用于评价嗜酸性粒细胞。免疫组织化学(IHC)染色和实时PCR用于评估小鼠鼻粘膜中TXNIP的免疫标记和mRNA表达。用MDA和SOD检测试剂盒测定鼻腔组织匀浆中丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性。血清中OVA特异性IgE和组胺的浓度,和OVA特异性IgE,PGD2,LTC4,ECP,采用ELISA法检测鼻腔灌洗液(NLF)中IL-4、IL-5、IL-6、IL-33和TNF-α的含量。体外研究,西方印迹,实时PCR,ELISA,ROS检测染料DCFH-DA,MDA,和SOD测定试剂盒,以评估OVA的作用和机制,白藜芦醇或NAC对脾单核细胞。
■我们发现了喷嚏的显着变化,擦鼻,炎性细胞因子,嗜酸性粒细胞数量,TXNIP,MDA,与未处理的AR小鼠相比,白藜芦醇或NAC处理的小鼠中的SOD水平。在培养的脾单核细胞中,TXNIP,MDA,SOD,OVA改变了ROS和炎性细胞因子水平,但白藜芦醇或NAC逆转了ROS和炎性细胞因子水平。
■白藜芦醇可通过抑制TXNIP-氧化应激通路有效缓解小鼠AR。
UNASSIGNED: Allergic rhinitis (AR) is a type I hypersensitivity mediated by IgE in the nose. Thioredoxin-interacting protein (TXNIP) plays a pivotal role in the process of producing reactive oxygen species (ROS). Resveratrol is a TXNIP inhibitor. Nonetheless, its role and mechanism in AR are still undetermined. The present study aimed to explore the effect and mechanism of resveratrol on an ovalbumin (OVA) induced mouse model of AR.
UNASSIGNED: AR murine model was established using OVA and administrated intranasally with resveratrol or N-acetylcysteine (NAC). Hematoxylin and eosin (HE) stain was used for evaluating eosinophils. Immunohistochemistry (IHC) staining and real-time PCR were employed to evaluate immunolabeling and mRNA expression of TXNIP in nasal mucosas of mice. Malondialdehyde (MDA) level and superoxide dismutase (SOD) activity in nasal tissue homogenates were measured using MDA and SOD Assay Kit. Concentrations of OVA-specific IgE and histamines in serum, and OVA-specific IgE, PGD2, LTC4, ECP, IL-4, IL-5, IL-6, IL-33 and TNF-α in nasal lavage fluid (NLF) were assayed by ELISA. In vitro studies, western blotting, real-time PCR, ELISA, ROS detecting dye DCFH-DA, MDA, and SOD Assay Kit were performed to evaluate the effects and mechanisms of OVA, resveratrol or NAC on spleen mononuclear cells.
UNASSIGNED: We found significant alternations of sneezing, nasal rubbing, inflammatory cytokines, eosinophil numbers, TXNIP, MDA, and SOD levels in resveratrol or NAC treated mice compared with untreated AR mice. In cultured spleen mononuclear cells, TXNIP, MDA, SOD, ROS and inflammatory cytokines levels were altered by OVA but reversed by resveratrol or NAC.
UNASSIGNED: Resveratrol could effectively alleviate murine AR by inhibiting TXNIP-oxidative stress pathway.