目的:表位H在特定的构象或环境中含有O-连接的N-乙酰葡糖胺(O-GlcNAcH)残基,由位点特异性单克隆小鼠IgM抗体H.O-GlcNAcH发生在几种正常和病理细胞以及几种多肽中,包括角蛋白-8和波形蛋白,在后者的细胞压力下。
方法:在这项工作中,我们研究了60例宫颈刮片标本中O-GlcNAcH在宫颈黏膜细胞上的分布,其中10个包含15个发炎的息肉。
结果:在我们的结果中,粘膜中O-GlcNAcH的表达较弱,粘蛋白分泌细胞<5%,息肉染色阳性高达30%。所有非纤毛,非粘蛋白分泌细胞,正常和增生的“储备”细胞,以及未成熟鳞状上皮化生的细胞,显示O-GlcNAcH的强弥漫性细胞质染色。在成熟鳞状上皮中,少于5%的基底细胞和所有中间和浅表细胞显示O-GlcNAcH的细胞质染色,而副基底细胞为阴性。所有纤毛细胞均呈斑片状或弥漫性细胞质染色。O-GlcNAcH的核染色较弱,增生性“储备”不到5%,纤毛细胞染色呈阳性。此外,粘膜成纤维细胞阴性,而息肉的所有基质细胞均显示O-GlcNAcH的强细胞质染色。
结论:O-GlcNAcH是:a)在粘膜和息肉的细胞元件中差异表达,b)在息肉的粘蛋白分泌细胞中上调,c)在发炎息肉的基质细胞中诱导,和d)可用作区分“储备”(阳性)和副基底(阴性)细胞的标记,使用常规细胞学染色具有相似的形态。
OBJECTIVE: Epitope H contains an O-linked N-acetylglucosamine (O-GlcNAcH) residue in a specific conformation or environment, recognized by a site-specific monoclonal mouse IgM antibody H. O-GlcNAcH occurs in several normal and pathological cells and in several polypeptides, including keratin-8 and vimentin, on the latter in cells under stress.
METHODS: In this work, we studied the distribution of O-GlcNAcH on cells of endocervical mucosa in 60 specimens of endocervical curettings, 10 of which contained 15 inflamed polyps.
RESULTS: In our results, expression of O-GlcNAcH was weak in the mucosa with <5% mucin-secreting cells and up to 30% of the polyps staining positively. All non-ciliated, non-mucin-secreting cells, normal and hyperplastic \'reserve\' cells, as well as the cells of immature squamous metaplasia, showed strong diffuse cytoplasmic staining for O-GlcNAcH. In mature squamous epithelium, fewer than 5% of basal cells and all the intermediate and superficial cells showed cytoplasmic staining for O-GlcNAcH, whereas parabasal cells were negative. All ciliated cells showed patchy or diffuse cytoplasmic staining. Nuclear staining for O-GlcNAcH was weak with fewer than 5% of hyperplastic \'reserve\' and ciliated cells staining positively. Moreover, mucosal fibroblasts were negative, whereas all stromal cells of the polyps showed strong cytoplasmic staining for O-GlcNAcH.
CONCLUSIONS: O-GlcNAcH is: a) differentially expressed among the cellular elements of mucosa and polyps, b) upregulated in mucin-secreting cells of polyps, c) induced in stromal cells of inflamed polyps, and d) can be used as a marker to differentiate between \'reserve\' (positive) and parabasal (negative) cells, which have similar morphology using conventional cytological stains.