%0 Journal Article
%T Integrating HexNAcQuest with Glycoproteomics Data Analysis Software to Distinguish HexNAc Isomers on Peptides.
%A Hou C
%A Li W
%A Li Y
%A Ma J
%J Methods Mol Biol
%V 2836
%N 0
%D 2024
%M 38995536
暂无%R 10.1007/978-1-0716-4007-4_5
%X Recently, HexNAcQuest was developed to help distinguish peptides modified by HexNAc isomers, more specifically O-linked β-N-acetylglucosamine (O-GlcNAc) and O-linked α-N-acetylgalactosamine (O-GalNAc, Tn antigen). To facilitate its usage (particularly for datasets from glycoproteomics studies), herein we present a detailed protocol. It describes example cases and procedures for which users might need to use HexNAcQuest to distinguish these two modifications.