■最近,口腔微生物与口腔鳞状细胞癌(OSCC)发生和进展相关的可能性已引起关注;这种联系还不清楚。这里,我们使用下一代测序技术全面分析唾液样本的微生物组谱,然后确定口腔微生物组谱与OSCC之间的关联.
■OSCC患者唾液样本中的微生物组分布,口腔白斑(OLK),术后OSCC(Post)进行分析。通过比较基于这些微生物组的各组的细菌多样性和相对丰度来鉴定候选OSCC相关细菌。并对其作为OSCC检测工具的适用性进行了评估。
■属丰度存在显着差异(链球菌,Aggregatibacter,和Alloprevotella)在唾液样本中。在OSCC组中,与OLK和邮政组相比,梭菌属的丰度,镰刀菌门和拟杆菌门显著增加,链球菌和厚壁菌门的数量减少。
■结果表明这些细菌与OSCC有很强的相关性。尤其是,梭菌门与OSCC早期复发显著相关。因此,口腔微生物组分析可能具有新型OSCC检测和预后工具的潜力.
UNASSIGNED: Recently, the possibility that oral microbiomes is associated with oral squamous cell carcinoma (OSCC) initiation and progression has attracted attention; however, this association is still unclear. Here, we comprehensively analyze the microbiome profiles of saliva samples using next-generation sequencing followed by determining the association between oral microbiome profiles and OSCC.
UNASSIGNED: Microbiome profiles in saliva samples from patients with OSCC, oral leukoplakia (OLK), and postoperative OSCC (Post) were analyzed. Candidate OSCC-associated bacteria were identified by comparing the bacterial diversity and relative abundance of each group based on these microbiome profiles, and their applicability as OSCC detection tools were evaluated.
UNASSIGNED: There were significant differences in genus abundances (Streptococcus, Aggregatibacter, and Alloprevotella) among the groups from saliva samples. In the OSCC group, compared with the OLK and Post groups, abundances of the genus Fusobacterium, phylum Fusobacteria and phylum Bacteroidetes were markedly increased and that of the genus Streptococcus and phylum Firmicutes were decreased.
UNASSIGNED: The results suggested a strong association of these bacteria with OSCC. Especially, phylum Fusobacterium was significantly associated with early recurrence of OSCC. Thus, oral microbiome analysis may have a potential of novel OSCC detection and prognostic tool.