{Reference Type}: Journal Article
{Title}: Evaluation of parentage testing using single nucleotide polymorphism markers for draft horses in Japan.
{Author}: Ishige T;Kikuchi M;Kakoi H;Hirota KI;Ohnuma A;Tozaki T;Hirosawa Y;Tanaka S;Nagata SI;
{Journal}: Anim Sci J
{Volume}: 94
{Issue}: 1
{Year}: 2023 Jan-Dec
{Factor}: 1.974
{DOI}: 10.1111/asj.13854
{Abstract}: We evaluated the utility of single nucleotide polymorphism (SNP) markers for parentage testing in Breton (BR) and Percheron (PR) horses in Japan using the proposed International Society for Animal Genetics (P-ISAG) 147 SNP panel and 414 autosomal SNPs. Genomic DNA was extracted from 98 horses of two breeds, BR (n = 47) and PR (n = 51), and sequenced using next-generation sequencing. The average minor allele frequencies for the P-ISAG panel for BR and PR were 0.306 and 0.301, respectively. The combined probabilities of exclusion (PEs) given two parents and one offspring: exclude a relationship (PE01) and given one parent and one offspring: exclude their relationship (PE02) were over 0.9999 for both breeds. Using the P-ISAG panel, no exclusion or doubtful cases were identified in 35 valid parent-offspring pairs, suggesting that the P-ISAG panel is helpful for parentage verification in both breeds. In contrast, as 0.18% of falsely accepted parentages were observed in the parentage discovery cases, additional markers such as the combination of the P-ISAG panel and 414 autosomal SNPs (561-SNP set) presented here should be used to identify valid parent-offspring pairs of horses with unknown parentage relationships.