背景:口腔炎症,通常由感染引发,受伤,或者免疫反应,会损害治疗结果,延迟愈合,并导致患者不适。绿色纳米粒子合成方法的发展由于其优于现有方法的潜在优势而受到关注。这些程序使用通常可用,负担得起的,和环保的天然植物提取物。由于它们在各个行业的众多用途,氧化钛纳米粒子(TiO2NPs)在纳米粒子中吸引了最多的注意力。在这项研究中,我们介绍了肉豆蔻(Mace)提取物作为还原剂和稳定剂的绿色合成,用于生产姜黄素官能化的TiO2NP(CTN)。我们还评估了这些纳米颗粒作为抗炎剂的有效性。
目的:在本研究中,我们的目标是开发生物TiO2NP,使用肉豆蔻作为天然封端剂,并与姜黄素功能化,以有效地管理口腔炎症在牙科应用。
方法:使用绿色合成方法合成纳米颗粒,并使用各种表征技术进行表征。使用溶血试验评估生物相容性,并使用抗炎试验评估纳米颗粒的生物活性。
结果:成功合成了姜黄素涂层的M-TiO2NP(MCTN),并通过各种技术进行了表征,确认它们的形态,结晶度功能化,元素组成,尺寸,和稳定性。体外生物活性研究表明,MCTN表现出显著的抗炎活性,如通过具有最小溶血潜力的蛋白质变性的抑制所证明的。这些发现突出了MCTN作为抗炎应用的有希望的候选物的潜力。
结论:我们的结果表明,MCTN具有良好的抗炎和抗溶血特性。然而,需要进一步深入的体内分析,以充分了解其功效和毒性。
BACKGROUND: Oral inflammation, often triggered by infections, injuries, or immune responses, can compromise treatment outcomes, delay healing, and contribute to patient discomfort. The development of green nanoparticle synthesis methods is receiving attention due to their potential advantages over existing approaches. These procedures use commonly available, affordable, and environmentally friendly natural plant extracts. Due to their numerous uses in various industries, titanium oxide nanoparticles (TiO2NPs) have attracted the most attention among the nanoparticles. In this study, we present the green synthesis of Myristica fragrans (mace) extract as a reductant and stabilizer for the production of curcumin-functionalized TiO2NPs (CTN). We additionally evaluated the effectiveness of these nanoparticles as anti-inflammatory agents.
OBJECTIVE: In this study, we aim to develop biogenic TiO2NPs using Myristica fragrans as a natural capping agent and functionalized with curcumin for effectively managing oral inflammation in dental applications.
METHODS: The nanoparticles were synthesized using the green synthesis method and characterized using various characterization techniques. Biocompatibility was evaluated using hemolytic assays, and the bioactivity of the nanoparticles was assessed using anti-inflammatory assays.
RESULTS: Curcumin-coated M-TiO2NPs (MCTN) were successfully synthesized and characterized by various techniques, confirming their morphology, crystallinity, functionalization, elemental composition, size, and stability. In vitro bioactivity studies revealed that MCTN exhibited significant anti-inflammatory activity, as evidenced by the inhibition of protein denaturation with minimal hemolytic potential. These findings highlight the potential of MCTN as a promising candidate for anti-inflammatory applications.
CONCLUSIONS: Our results suggest that MCTN exhibits promising anti-inflammatory and anti-hemolytic properties. However, further in-depth in vivo analysis is required to fully understand their efficacy and toxicity.