Abstract：
The bZIP transcription factors play crucial roles in various aspects of plant biology, including development, defence mechanisms, senescence, and responses to both biotic and abiotic environmental stresses. Myristica fragrans Houtt. transcriptome analysis has identified 15 bZIP transcription factors, each exhibiting major conserved domains and motifs such as BRLZ, MFMR, and DOG1. Functional characterisation of these identified MfbZIP factors indicates their predominant localisation within the nucleus. Phylogenetic analysis reveals that MfbZIP factors cluster into three subgroups alongside annotated bZIP sequences from Magnolia sinica and Arabidopsis thaliana. Moreover, gene ontology (GO) analysis highlights several key functions of MfbZIP, including involvement in defence responses, abscisic acid-induced signalling pathways, and DNA-binding transcription factor activity. Further investigation through KEGG pathway analysis reveals that the amino acid sequences of MfbZIP contain binding motifs for proteins such as TGA, implicated in plant hormone signal transduction pathways associated with disease resistance. To confirm the disease-defence-related activity of the TGA binding protein within MfbZIP, we employed amino acid sequences for 3-D ab initio modelling. Subsequently, we analysed TGA-NPR1 interactions using docking and molecular dynamics simulation analysis. These analyses shed light on the functional and structural aspects of TGA, demonstrating its stable association with NPR1 protein and its significance in the expression of PR1 protein, thus playing a pivotal role in defence responses against pathogens.
摘要：
bZIP转录因子在植物生物学的各个方面发挥着至关重要的作用，包括发展,防御机制,衰老，以及对生物和非生物环境胁迫的反应。MyristicafragransHoutt。转录组分析已经确定了15个bZIP转录因子，每个都表现出主要的保守域和基序，如BRLZ，MFMR,DOG1这些鉴定的MfbZIP因子的功能表征表明它们在细胞核内的主要定位。系统发育分析表明，MfbZIP因子与来自木兰和拟南芥的带注释的bZIP序列一起聚集到三个亚组中。此外，基因本体论(GO)分析突出了MfbZIP的几个关键功能，包括参与防御反应，脱落酸诱导的信号通路，和DNA结合转录因子活性。通过KEGG通路分析的进一步研究表明，MfbZIP的氨基酸序列含有TGA等蛋白质的结合基序，与抗病性相关的植物激素信号转导途径。为了确认MfbZIP中TGA结合蛋白的疾病防御相关活性，我们使用氨基酸序列进行3-D从头算建模。随后，我们使用对接和分子动力学模拟分析分析了TGA-NPR1相互作用。这些分析揭示了TGA的功能和结构方面，证明其与NPR1蛋白的稳定关联及其在PR1蛋白表达中的意义，因此在抵抗病原体的防御反应中起着关键作用。
