目的:为了评估生物相容性,生物活性,三种新型硅酸钙水泥基密封剂的免疫调节性能:Ceraseal(CS),完全填充BC密封剂(TFbc)和WellRootST(WR-ST)对人牙周膜干细胞(hPDLSC)的影响。
方法:从健康患者的第三磨牙中分离HPDLSCs。洗脱液(1:1、1:2和1:4比例)和CS样品盘,准备固化后的TFbc和WR-ST。进行了一系列测定:细胞表征,细胞代谢活性(MTT测定)细胞附着和形态学(SEM测定),细胞迁移(伤口愈合试验),细胞骨架组织(基于phaloidin的测定);IL-6和IL-8释放(ELISA);分化标记表达(RT-qPCR测定),和细胞矿化(茜素红S染色)。在非条件(阴性对照)或成骨(阳性对照)培养基中培养的HPDLSC用作比较。在p<0.05时确立统计学显著性。
结果:所有测试的封闭剂在细胞相容性测定(细胞代谢活性,迁移,附件,形态学,和细胞骨架组织)与阴性对照组相比。与阴性和阳性对照组相比,CS和TFbc显示至少一种成骨/骨水泥标志物的上调。CS和TFbc也显示出明显高于阴性和阳性对照组的钙化结节形成。CS处理的细胞中的标志物表达和钙化结节形成均明显高于TFbc处理的细胞。WR-ST表现出与对照组相似的结果。与阴性对照组相比,CS和TFbc处理的细胞在培养72小时后表现出IL-6的显著下调(p<0.05)。
结论:所有测试的封闭剂都表现出足够的细胞相容性。CS通过上调与骨和牙骨质形成相关的关键基因的表达而显着增强细胞分化。此外,观察到CS有效地促进细胞外基质的矿化。相比之下,与CS相比,TFbc和WR-ST对这些过程的影响不那么明显。此外,CS和TFbc均表现出抗炎潜力,有助于它们在再生牙髓中的潜在治疗益处。
结论:这是第一个比较脑膜生物学特性和免疫调节潜能的研究,完全填充BC密封剂,和WellRootST。结果为其在根管治疗中的使用提供了支持证据。
OBJECTIVE: To assess the biocompatibility, bioactivity, and immunomodulatory properties of three new calcium silicate cement-based sealers: Ceraseal (CS), Totalfill BC Sealer (TFbc) and WellRoot ST (WR-ST) on human periodontal ligament stem cells (hPDLSCs).
METHODS: HPDLSCs were isolated from extracted third molars from healthy patients. Eluates (1:1, 1:2, and 1:4 ratio) and sample discs of CS, TFbc and WR-ST after setting were prepared. A series of assays were performed: cell characterization, cell metabolic activity (MTT assay) cell attachment and morphology (SEM assay), cell migration (wound-healing assay), cytoskeleton organization (phaloidin-based assay); IL-6 and IL-8 release (ELISA); differentiation marker expression (RT-qPCR assay), and cell mineralization (Alizarin Red S staining). HPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media were used as a comparison. Statistical significance was established at p < 0.05.
RESULTS: All the tested sealers exhibited similar results in the cytocompatibility assays (cell metabolic activity, migration, attachment, morphology, and cytoskeleton organization) compared with a negative control group. CS and TFbc exhibited an upregulation of at least one osteo/cementogenic marker compared to the negative and positive control groups. CS and TFbc also showed a significantly higher calcified nodule formation than the negative and positive control groups. Both the marker expression and calcified nodule formation were significantly higher in CS-treated cells than TFbc treated cells. WR-ST exhibited similar results to the control group. CS and TFbc-treated cells exhibited a significant downregulation of IL-6 after 72 h of culture compared to the negative control group (p < 0.05).
CONCLUSIONS: All the tested sealers exhibited an adequate cytocompatibility. CS significantly enhances cell differentiation by upregulating the expression of key genes associated with bone and cementum formation. Additionally, CS was observed to facilitate the mineralization of the extracellular matrix effectively. In contrast, the effects of TFbc and WR-ST on these processes were less pronounced compared to CS. Furthermore, both CS and TFbc exhibited an anti-inflammatory potential, contributing to their potential therapeutic benefits in regenerative endodontics.
CONCLUSIONS: This is the first study to compare the biological properties and immunomodulatory potential of Ceraseal, Totalfill BC Sealer, and WellRoot ST. The results act as supporting evidence for their use in root canal treatment.