迫切需要了解与电子烟相关的健康风险,在青少年中已经达到流行水平。Juul是目前市场上最受欢迎的电子烟。使用比较毒性基因组学数据库(CTD;http://ctdbase.org),整合化学的公共资源,基因,表型和疾病数据,我们旨在分析加热Juul电子烟盒产生的气溶胶中检测到的八种化学物质的潜在分子机制:尼古丁,乙醛,甲醛,自由基,巴豆醛,丙酮,丙酮酸,和颗粒物.CTD中策划的内容,包括化学基因,化学表型,和化学疾病的相互作用,以及相关的表型和途径富集,进行了分析,以帮助确定与电子烟相关的潜在分子机制和疾病。尼古丁显示了这些化学物质最直接的疾病关联,其次是颗粒物和甲醛.一起,这些化学物质显示出与CTD中400种独特疾病的直接标记或机制关系,特别是在心血管疾病类别中,神经系统疾病,呼吸道疾病,癌症,和精神障碍。我们选择了三种呼吸道疾病来进一步研究,并发现除了细胞凋亡和细胞增殖的细胞过程,Juul相关呼吸道疾病结果的优先表型包括对氧化应激的反应,炎症反应,和几种细胞信号通路(p38MAPK,NIK/NFkappaB,钙介导的)。
There is a critical need to understand the health risks associated with vaping e-cigarettes, which has reached epidemic levels among teens. Juul is currently the most popular type of e-cigarette on the market. Using the Comparative Toxicogenomics Database (CTD; http://ctdbase.org), a public resource that integrates chemical, gene, phenotype and disease data, we aimed to analyze the potential molecular mechanisms of eight chemicals detected in the aerosols generated by heating Juul e-cigarette pods: nicotine, acetaldehyde, formaldehyde, free radicals, crotonaldehyde, acetone, pyruvaldehyde, and particulate matter. Curated content in CTD, including chemical-gene, chemical-phenotype, and chemical-disease interactions, as well as associated phenotypes and pathway enrichment, were analyzed to help identify potential molecular mechanisms and diseases associated with vaping. Nicotine shows the most direct disease associations of these chemicals, followed by particulate matter and formaldehyde. Together, these chemicals show a direct marker or mechanistic relationship with 400 unique diseases in CTD, particularly in the categories of cardiovascular diseases, nervous system diseases, respiratory tract diseases, cancers, and mental disorders. We chose three respiratory tract diseases to investigate further, and found that in addition to cellular processes of apoptosis and cell proliferation, prioritized phenotypes underlying Juul-associated respiratory tract disease outcomes include response to oxidative stress, inflammatory response, and several cell signaling pathways (p38MAPK, NIK/NFkappaB, calcium-mediated).