目的:通过评估血液生化,评估将银纳米粒子涂层微型植入物植入Wistar白化病大鼠作为动物模型的比较研究时,评估其全身毒性的可能性,肝肾功能,和植入部位的组织学。
方法:微型植入物的表面涂有绿色介导的银纳米颗粒。将未涂覆的微型植入物放置在两组八只Wistar白化病大鼠中,和银纳米粒子涂层的微型植入物被放置在另外八只大鼠。骨头的一般情况,血液生化评估ALT,AST,GPT,有,每隔7、14和28天检查一次使用H和E染色和Masson三色染色的组织学切片。
结果:肌酐,尿素,ALP,在测试组和对照组的Wistar大鼠中,ALT在28天的随访期内均未显示出全身毒性的迹象。组织学评估,这是使用HE和MTS染色进行的,在放置涂层微型植入物的组中,显示成骨作用和插入部位的充分愈合。在未涂覆的微型植入物的对照组中,骨样本没有异常。
结论:绿色合成的银纳米颗粒涂层微型植入物不会引起全身毒性,如肌酐水平无异常所示,尿素,ALT,ALP,GPT,和得到了。骨组织学表明,放置在动物骨中的涂覆微型植入物在足够的成骨作用下愈合。
结论:银纳米颗粒具有抗微生物活性的潜力。在正畸中作为临时锚固装置放置的微型植入物通常由于炎症和斑块而失败。银纳米颗粒涂覆的微型植入物将降低微型植入物失败的风险,因为它将具有抗微生物潜力并消除微型植入物失败的原因。如何引用这篇文章:SreenivasaganS,SubramanianAK,MohanrajKG,etal.绿色合成银纳米颗粒涂层钛微型植入物与未涂层微型植入物的毒性评估:动物模型研究中的比较。JContempDentPract2023;24(12):944-950。
OBJECTIVE: To assess the potential for systemic toxicity when silver nanoparticle-coated mini-implants were implanted in Wistar albino rats conducted as a comparative study in the animal model by assessing the blood biochemistry, liver and kidney function, and histology of the implanted site.
METHODS: The surface of the mini-implant was coated with a green-mediated silver nanoparticle. Uncoated mini-implants were placed in two groups of eight Wistar albino rats, and silver nanoparticle-coated mini-implants were placed in another eight rats. The bone\'s general conditions, blood biochemistry assessing for ALT, AST, GPT, GOT, and histological sections using H and E stain and Masson\'s Trichrome stain were examined at 7, 14, and 28-day intervals.
RESULTS: The creatinine, urea, ALP, and ALT showed no signs of systemic toxicity during the 28-day follow-up period in the Wistar rats both in the test and control groups. The histological evaluation, which was conducted using HE and MTS stain, revealed osteogenesis and adequate healing of the insertion site in the group where coated mini-implant was placed. The bone sample revealed no abnormalities in the control group with uncoated mini-implants.
CONCLUSIONS: Green synthesized silver nanoparticle-coated mini-implant does not cause systemic toxicity as indicated by no abnormalities in the levels of creatinine, urea, ALT, ALP, GPT, and GOT. The bone histology indicates that the coated mini-implants placed in animal bone healed with adequate osteogenesis.
CONCLUSIONS: Silver nanoparticles have potential for antimicrobial activity. Mini-implants placed as temporary anchorage devices in orthodontics often fail due to inflammation and plaque. Silver nanoparticle-coated mini-implants would reduce the risk of mini-implant failure as it would have antimicrobial potential and eliminate this cause for failure of mini-implants. How to cite this article: Sreenivasagan S, Subramanian AK, Mohanraj KG, et al. Assessment of Toxicity of Green Synthesized Silver Nanoparticle-coated Titanium Mini-implants with Uncoated Mini-implants: Comparison in an Animal Model Study. J Contemp Dent Pract 2023;24(12):944-950.