在拟南芥中,绞股蓝,花的内圈,是女性的生殖部分。许多对受精很重要的组织,如柱头,风格,发射道,胎盘,胚珠,和隔膜,包括中间域,起源于心皮边缘分生组织。在绞股蓝发育过程中,发生隔膜融合,组织不断形成,为成功的授粉和受精做准备。在绞股蓝发育过程中,发生细胞壁修饰,最重要的是传输道的形成,对生殖能力有很大影响,因为它有助于花粉管的生长和通过卵巢的运动。在这项研究中,使用经典染色方法的组合,荧光染料,和间接免疫定位,我们分析了在雌蕊发育过程中伴随内侧结构域形成的细胞壁组成和修饰。我们检测到多糖分布随时间的协调变化,在传输道形成之前的细胞壁修饰,粘液物质在传播道形成过程中增加,甘露聚糖分布减少。此外,我们还检测到隔膜融合过程中脂质分布的变化.正确的细胞壁组成和修饰对于心皮的生殖器后融合(隔膜融合)和传输道的形成很重要,因为这些组织影响植物的繁殖能力。
In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part. Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem. During gynoecium development, septum fusion occurs and tissues form continuously to prepare for a successful pollination and fertilization. During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary. In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development. We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution. Furthermore, we also detected changes in lipid distribution during septum fusion. Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.