PDF(Pubmed)
Abstract：
Gene regulatory networks are now at the forefront of precision biology, which can help researchers better understand how genes and regulatory elements interact to control cellular gene expression, offering a more promising molecular mechanism in biological research. Interactions between the genes and regulatory elements involve different promoters, enhancers, transcription factors, silencers, insulators, and long-range regulatory elements, which occur at a ∼10 µm nucleus in a spatiotemporal manner. In this way, three-dimensional chromatin conformation and structural biology are critical for interpreting the biological effects and the gene regulatory networks. In the review, we have briefly summarized the latest processes in three-dimensional chromatin conformation, microscopic imaging, and bioinformatics, and we have presented the outlook and future directions for these three aspects.
摘要：
基因调控网络现在处于精准生物学的前沿,这可以帮助研究人员更好地了解基因和调控元件如何相互作用以控制细胞基因表达，在生物学研究中提供了更有前途的分子机制。基因和调控元件之间的相互作用涉及不同的启动子，增强器,转录因子，消音器,绝缘子,和远程监管元素，它们以时空方式发生在10µm的核上。这样,三维染色质构象和结构生物学对于解释生物效应和基因调控网络至关重要。在审查中,我们简要总结了三维染色质构象的最新过程，显微成像,和生物信息学，我们对这三个方面提出了展望和未来方向。
