气候变化是全球利益攸关方日益关注的问题。肠道受到热应激的严重影响。本研究旨在探讨蛋氨酸对热应激所致小鼠肠道损伤的缓解作用。将小鼠分为四组:对照组(C),蛋氨酸缺乏组(MD),蛋氨酸+热应激组(MH),和蛋氨酸缺乏+热应激组(MDH)。组织病理学技术,PAS-Alcian蓝染色,免疫组织化学方法,生化定量方法,ELISA,和显微方法用于研究肠粘膜形态的变化,杯状细胞的数量,紧密连接蛋白的表达,过氧化物产品含量和抗氧化酶活性,肠粘膜损伤,免疫球蛋白和HSP70的含量,Na/K-ATPase的活性。结果表明,蛋氨酸可以改善肠粘膜形态(增加绒毛高度,V/C值,和肌肉层厚度,减小隐窝深度),增加紧密连接蛋白的表达(Claudin-1,Occludin,ZO-1)和DAO的内容,降低肠粘膜损伤标志物(ET,FABP2)和过氧化产物(MDA),增加抗氧化酶的活性(GR,GSH-Px,SOD),杯状细胞的数量,免疫球蛋白的含量(sIgA,IgA,IgG,IgM)和应激蛋白(HSP70),和Na+/K+-ATP酶的活性。提示蛋氨酸可以减轻热应激小鼠的肠道损伤。
Climate change is an increasing concern of stakeholders worldwide. The intestine is severely impacted by the heat stress. This study aimed to investigate the alleviating effects of
methionine on the intestinal damage induced by heat stress in mice. The mice were divided into four groups: control group (C),
methionine deficiency group (MD),
methionine + heat stress group (MH), and
methionine deficiency + heat stress group (MDH). Histopathological techniques, PAS-Alcian blue staining, immunohistochemistry method, biochemical quantification method, ELISA, and micro method were used to study the changes in the intestinal mucosal morphology, the number of goblet cells, the expression of tight junction proteins, the peroxide product contents and antioxidant enzyme activities, the intestinal mucosal damage, the content of immunoglobulins and HSP70, the activity of Na+/K+-ATPase. The results showed that
methionine can improve intestinal mucosal morphology (increase the villi height, V/C value, and muscle layer thickness, decrease crypt depth), increase the expression of tight junction proteins (Claudin-1, Occludin, ZO-1) and the content of DAO, decrease the content of intestinal mucosa damage markers (ET, FABP2) and peroxidation products (MDA), increase the activity of antioxidant enzymes (GR, GSH-Px, SOD), the number of goblet cells, the contents of immunoglobulins (sIgA, IgA, IgG, IgM) and stress protein (HSP70), and the activity of Na+/K+-ATPase. It is suggested that
methionine can alleviate intestinal damage in heat-stressed mice.