由于其组织特异性表达特征,RNA在法医体液/组织鉴定(BFID)中引起了广泛关注。在RNA中,长RNA(例如,mRNA)具有包含更多多态性位点的更高概率,可用于分配体液/组织的特定供体。然而,很少有研究描述了他们在法医学中的整体概况。在这项研究中,我们对30个静脉血样本的转录组进行了测序,经血,精液,唾液,阴道分泌物,和皮肤组织,获得mRNA的全面图片,lncRNA,和circRNA配置文件。总共90,305个mRNA,102,906个lncRNAs(包括19,549个新lncRNAs),并检测到40,204个circRNAs。RNA类型分布,长度分布,并根据它们的注释和表达水平呈现表达分布,并鉴定了许多新的体液/组织特异性RNA标记。此外,根据基因注释分析了三种RNA之间的同源关系。最后,对来自RNA转录本的SNP和InDels进行基因分型,并鉴定了21,611个多SNP和4,471个多InDel转录组微单倍型(tMHs)。这些结果提供了对转录组概况的全面理解,这可以为追踪体液/组织的起源和识别个体提供新的途径。
RNAs have attracted much attention in forensic body fluid/tissue identification (BFID) due to their tissue-specific expression characteristics. Among RNAs, long RNAs (e.g., mRNA) have a higher probability of containing more polymorphic sites that can be used to assign the specific donor of the body fluid/tissue. However, few studies have characterized their overall profiles in forensic science. In this study, we sequenced the transcriptomes of 30 samples from venous blood, menstrual blood, semen, saliva, vaginal secretion, and skin tissue, obtaining a comprehensive picture of mRNA, lncRNA, and circRNA profiles. A total of 90,305 mRNAs, 102,906 lncRNAs (including 19,549 novel lncRNAs), and 40,204 circRNAs were detected. RNA type distribution, length distribution, and expression distribution were presented according to their annotation and expression level, and many novel body fluid/tissue-specific RNA markers were identified. Furthermore, the cognate relations among the three RNAs were analyzed according to gene annotations. Finally, SNPs and InDels from RNA transcripts were genotyped, and 21,611 multi-SNP and 4,471 multi-InDel transcriptomic microhaplotypes (tMHs) were identified. These results provide a comprehensive understanding of transcriptome profiles, which could provide new avenues for tracing the origin of the body fluid/tissue and identifying an individual.