Abstract：
Parkinson\'s disease is characterized by the progressive loss of dopaminergic neurons in the nigrostriatal pathway and oxidative stress is one of the main mechanisms that lead to neuronal death in this disease. Previous studies have shown antioxidant activity from the leaves of Byrsonima sericea, a plant of the Malpighiaceae family. This study aimed to evaluate the cytoprotective activity of the B. sericea ethanolic extract (BSEE) against the cytotoxicity induced by 6-hydroxydopamine (6-OHDA) in PC12 cells, an in vitro model of parkinsonism. The identification of phenolic compounds in the extract by HPLC-DAD revealed the presence of geraniin, rutin, isoquercetin, kaempferol 3-O-β-rutinoside, and quercetin. The BSEE (75-300 µg/mL) protected PC12 cells from toxicity induced by 6-OHDA (25 µg/mL), protected cell membrane integrity and showed antioxidant activity. BSEE was able to decrease nitrite levels, glutathione depletion, and protect cells from 6-OHDA-induced apoptosis. Thus, we suggest that the BSEE can be explored as a possible cytoprotective agent for Parkinson\'s disease due to its high antioxidant capacity and anti-apoptotic action.
摘要：
帕金森病的特征是黑质纹状体途径中多巴胺能神经元的进行性丢失,氧化应激是导致该疾病神经元死亡的主要机制之一。先前的研究表明，来自Byrsonimasericea叶子的抗氧化活性，一种马氏科植物。本研究旨在评估血清双歧杆菌乙醇提取物（BSEE）对PC12细胞中6-羟基多巴胺（6-OHDA）诱导的细胞毒性的细胞保护活性。帕金森病的体外模型.通过HPLC-DAD鉴定提取物中的酚类化合物揭示了香叶素的存在，芦丁,异槲皮素,山奈酚3-O-β-菊酯苷，还有槲皮素.BSEE（75-300µg/mL）保护PC12细胞免受6-OHDA（25µg/mL）诱导的毒性，保护细胞膜的完整性，并表现出抗氧化活性。BSEE能够降低亚硝酸盐水平，谷胱甘肽耗竭，并保护细胞免受6-OHDA诱导的凋亡。因此，我们认为，由于BSEE具有高抗氧化能力和抗凋亡作用，可以将其作为帕金森病的细胞保护剂。
