这项研究旨在调查从日本三级保健医院获得的肺炎球菌分离株的细菌特征。我们分析了抗菌药物的敏感性,拥有大环内酯抗性基因,肺炎球菌血清组/血清型,长崎大学医院2011年至2020年间15岁或以上患者肺炎球菌分离株的序列类型(ST)。在分析的73个分离株中,86.3%对大环内酯类抗生素有抗性,和28.8%,46.6%,11.0%的人携带mefA,ermB,两者,分别。在拥有ermB的分离物中,97.6%表现出高水平的大环内酯抗性[最小抑制浓度(MIC)范围,>16µg/mL]。索利霉素(MIC范围,0.03-0.25µg/mL),无论是否存在大环内酯抗性基因,和拉苏沙星(MIC范围,0.06-0.5µg/mL)显示出有效的体外抗肺炎球菌活性。血清型19A是最普遍的(六个分离株),其次是血清型10A,15A,和15B/C(各5个分离株)。四种血清型(11A,19A,22F,和23B)和五个ST(36、99、433、558和3111)与大环内酯抗性基因的存在显着相关。血清型11A/ST99的所有四个分离株和血清型19A/ST3111的三个分离株都携带mefA和ermB。在两个血清型为22F/ST433的分离株中均未检测到大环内酯抗性基因,而所有十个血清型为15的分离株(血清型15A和15B/C,每个五个分离株)仅拥有ermB。我们的研究揭示了从我们医院获得的肺炎球菌分离株的细菌特征。证实了索利霉素和拉库沙星对这些分离株的体外活性。
This study aimed to investigate the bacterial characteristics of pneumococcal isolates obtained from a tertiary care hospital in Japan. We analyzed the antimicrobial susceptibility, possession of macrolide resistance genes, pneumococcal serogroup/serotype, and sequence type (ST) of pneumococcal isolates from patients aged 15 years or older between 2011 and 2020 at Nagasaki University Hospital. Of the 73 isolates analyzed, 86.3% showed resistance to macrolides, and 28.8%, 46.6%, and 11.0% harbored mefA, ermB, and both, respectively. Of the isolates possessing ermB, 97.6% showed high levels of macrolide resistance [minimal inhibitory concentration (MIC) range, > 16 µg/mL]. Solithromycin (MIC range, 0.03-0.25 µg/mL), regardless of the presence of macrolide resistance genes, and lascufloxacin (MIC range, 0.06-0.5 µg/mL) showed potent in vitro activity against pneumococci. Serotype 19A was the most prevalent (six isolates), followed by serotypes 10A, 15A, and 15B/C (five isolates each). Four serotypes (11A, 19A, 22F, and 23B) and five STs (36, 99, 433, 558, and 3111) were significantly correlated with the presence of macrolide resistance genes. All four isolates with serotype 11A/ST99 and three isolates with serotype 19A/ST3111 harbored both mefA and ermB. No macrolide resistance genes were detected in either of the two isolates with serotype 22F/ST433, while all ten isolates with serogroup 15 (serotypes 15A and 15B/C, five isolates each) possessed ermB alone. Our study revealed the bacterial characteristics of the pneumococcal isolates obtained from our hospital. In vitro activity of solithromycin and lascufloxacin against these isolates was confirmed.