未经批准:梅毒螺旋体的大环内酯耐药性(T。pallidum)近年来在全球范围内一直在增加。然而,关于新疆梅毒螺旋体大环内酯类药物耐药性的数据很少,位于中国西部,这是梅毒发病率较高的地区。在这项研究中,我们调查了新疆潜伏梅毒患者梅毒螺旋体大环内酯耐药的分子特征,中国。
未经批准:总共,收集2016-2017年新疆医科大学第一医院隐性梅毒患者全血204份。使用QIAampDNAMini试剂盒提取血液样品的基因组DNA,并通过PCR检测T.pallidum的特定polA基因。通过巢式PCR在梅毒螺旋体阳性样品中扩增出梅毒螺旋体的23SrRNA基因,使用限制酶MboII和BsaI鉴定了23SrRNA基因中与大环内酯抗性相关的突变位点A2058G和A2059G。
未经授权:梅毒T.的特定polA基因(T.在204例潜伏梅毒患者的27份血液样本(13.2%)中检测到苍白球阳性)。然后在所有27个梅毒螺旋体阳性样品中扩增23SrRNA基因,其中24/27例(88.9%)在23SrRNA基因中存在A2058G突变,3/27例(11.1%)存在A2059G突变。
UNASSIGNED:我们的结果表明,新疆的梅毒大环内酯耐药不容忽视,中国,A2058G是主要的大环内酯抗性机制。血液可能是检测未显示任何临床症状的潜伏梅毒患者的梅毒螺旋体耐药突变的合适样本。
UNASSIGNED: Macrolide resistance in Treponema pallidum (T. pallidum) has been increasing in recent years worldwide. However, few data are available on macrolide resistance in T. pallidum from Xinjiang province, located in the western part of
China, which is an area with a high incidence of syphilis. In this study, we investigated the molecular characteristics of macrolide resistance in T. pallidum from patients with latent syphilis in Xinjiang,
China.
UNASSIGNED: In total, 204 whole blood samples were collected from patients with latent syphilis during 2016 to 2017, in the First Hospital of Xinjiang Medical University. Genomic DNA of blood samples was extracted using a QIAamp DNA Mini Kit and T. pallidum was detected by PCR with the specific polA gene of T. pallidum. The 23S rRNA gene of T. pallidum was amplified among the T. pallidum-positive samples by nested PCR, and macrolide resistance-associated mutation sites A2058G and A2059G in the 23S rRNA gene were identified using restriction enzymes MboII and BsaI.
UNASSIGNED: The specific polA gene of T. pallidum (T. pallidum positive) was detected in 27 blood samples (13.2%) from 204 patients with latent syphilis. The 23S rRNA gene was then amplified in all 27 T. pallidum-positive samples, among which 24/27 samples (88.9%) harbored the A2058G mutation in the 23S rRNA gene and 3/27 (11.1%) had the A2059G mutation.
UNASSIGNED: Our results indicated that T. pallidum macrolide resistance should not be ignored in Xinjiang,
China, and that A2058G was the predominant macrolide resistance mechanism. Blood may be a suitable specimen for the detection of resistant mutations of T. pallidum in patients with latent syphilis who do not show any clinical symptoms.