背景:许多酚类C-糖苷具有营养益处和药理作用。然而,酚类C-糖苷分析的MS/MS断裂模式研究不足。本文旨在确定酚类C-糖苷的MS/MS断裂模式。
方法:10种不同糖部分的化合物,苷元,并使用UPLC-QTOF-MS分析分析替代品以确定这些结构特征对MS/MS片段化的影响。
结果:结果表明,水的流失以及C-C键合的糖部分中的RDA反应和α裂解是主要的片段化途径。此外,糖裂解不受骨架和糖苷配基替代品的影响。这些结果表明,酚类C-糖苷的片段化模式与O-糖苷中的片段化模式不同,其中O-C糖苷键是MS/MS分析中最易于切割的键。
结论:这些MS/MS片段模式可用于从饮食成分和草药中鉴定C-糖苷,以及使用MRM方法开发可靠的方法来定量C-糖苷。
BACKGROUND: Many phenolic C-glycosides possess nutritional benefits and pharmacological efficacies. However, the MS/MS fragmentation pattern of phenolic C-glycosides analysis is understudied. This paper aims to determine the MS/MS fragmentation patterns of phenolic C-glycosides.
METHODS: Ten compounds with different sugar moieties, aglycones, and substitutes were analyzed to determine the impact of these structural features on MS/MS fragmentation using UPLC-QTOF-MS analysis.
RESULTS: The results showed that water loss followed by RDA reaction and alpha cleavage in the C-C bonded sugar moieties are the major fragmentation pathways. Additionally, the sugar cleavage was not affected by the skeleton and the substitute of the aglycones. These results suggested that the fragmentation patterns of phenolic C-glycosides differ from those in the O-glycosides, where the O-C glycosidic bond is the most cleavage-liable bond in MS/MS analysis.
CONCLUSIONS: These MS/MS fragmentation patterns can be used for the identification of C-glycosides from dietary components and herbal medicine as well as developing robust methods using MRM methods to quantify C-glycosides.