■巨噬细胞在调节炎症和组织再生中起重要作用。已知抗炎巨噬细胞在组织再生中起重要作用。这项研究的目的是通过利用药物递送技术来修饰巨噬细胞的表型以实现抗炎功能。
■在这项研究中,制备了4种类型的聚(L-乳酸-共-乙醇酸)(PLGA)微球,所述微球包含具有不同尺寸的抗炎调节剂(pio-MS)的吡格列酮。pio-MS的体外释放试验在含有1重量%十二烷基硫酸钠的磷酸盐缓冲盐溶液(PBS)中进行。评估了与pio-MS一起培养的小鼠骨髓源性巨噬细胞(BMDM)的精氨酸酶活性和作为抗炎巨噬细胞标记的白介素(IL)-10的分泌。
■在体外从所有类型的pio-MS观察到吡格列酮的持续释放。当BMDM与平均直径为40μm的pio-MS(pio-MS40)一起培养时,与其他pio-MS相比,精氨酸酶活性和IL-10的分泌显着增加。
■直径为40μm的pio-MS40具有在该培养系统中诱导BMDM抗炎修饰的潜力。吡格列酮的持续释放促进巨噬细胞功能的修饰。
UNASSIGNED: Macrophages play an important role in regulating inflammation and tissue regeneration. It is known that anti-inflammatory macrophages play an important role for tissue regeneration. The objective of this study is to modify macrophages phenotypes for anti-inflammatory function by utilizing drug delivery technology.
UNASSIGNED: In this study, 4 types of poly (L-lactic-co-glycolic acid) (PLGA) microspheres incorporating pioglitazone of an anti-inflammatory modifier (pio-MS) with different sizes were prepared. In vitro release test of pio-MS was performed in phosphate buffered-saline solution (PBS) containing 1 wt% of sodium lauryl sulfate. The arginase activity and the secretion of interleukin (IL)-10 as anti-inflammatory macrophage markers of mouse bone marrow derived-macrophages (BMDM) cultured with the pio-MS were evaluated.
UNASSIGNED: The sustained release of pioglitazone was observed from all types of pio-MS in vitro. When BMDM were cultured with the pio-MS with an average diameter of 40 μm (pio-MS40), the arginase activity and the secretion of IL-10 increased to a significant extent compared with other pio-MS.
UNASSIGNED: The pio-MS40 with an diameter of 40 μm had a potential to induce the anti-inflammatory modification of BMDM in this culture system. The sustained release of pioglitazone is promoting to modify the macrophage function.