忍冬素靶向 EZH2 通过自噬介导的 NLRP3 炎性体失活减轻溃疡性结肠炎.
关键词: 3-MC, 3-methylcholanthrene 5-ASA, 5-aminosalicylic acid AIM2, absent in melanoma 2 ATG5, autophagy-related protein 5 ATG7, autophagy-related protein 7 ATP, adenosine triphosphate Autophagy BMDMs, bone marrow-derived macrophages CETSA, cellular thermal shift assay CHX, cycloheximide ChIP, chromatin immunoprecipitation Colitis DAI, disease activity index DAMPs, damage-associated molecular patterns DMSO, dimethyl sulfoxide DSS, dextran sulfate sodium DTT, dithiothreitol ECL, enhanced chemiluminescent EDTA, ethylenediaminetetraacetic acid ELISA, enzyme-linked immunosorbent assay EZH2 EZH2, enhancer of zeste homolog 2 FBS, fetal bovine serum H&E, hematoxylin and eosin LPS, lipopolysaccharide Lonicerin M-CSF, macrophage colony stimulating factor MDP, muramyldipeptide MPO, myeloperoxidase MSU, monosodium urate crystals NLRP3 inflammasome NLRP3, nucleotide-binding domain-like receptors family pyrin domain containing 3 PAMPs, pathogen-associated molecular patterns PMA, phorbol myristate acetate PMSF, phenylmethanesulfonyl fluoride PRC2, polycomb repressive complex 2 RMSD, root mean-square deviation RMSF, root mean-square fluctuation SIP, solvent-induced protein precipitation TEM, transmission electron microscopy UC, ulcerative colitis
来 源:
DOI:10.1016/j.apsb.2021.03.011
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