心肌梗死(MI)引起的内脏器官损伤,虽然经常被忽视,是一种非常严重的疾病,会损害内脏器官,尤其是肺部。微循环的变化可以从急性肺损伤开始,并导致严重的呼吸衰竭。这项研究的目的是通过研究维生素D(VITD)和NRD(NRD)对MI引起的肺部损伤的治疗作用,创造新的方法来解释该疾病的病理生理学和治疗方法。以及它们与asprosin/spexin蛋白的关系。
方法:构成6组,每组7只实验动物。Control,VITD(实验期间仅50IU/天),NRD(实验期间仅100mg/kg/天),MI(200mg/kg异丙肾上腺素作为单次剂量皮下给予大鼠),MI+VITD(200mg/kg异丙肾上腺素+50IU/天)和MI+NRD(200mg/kg异丙肾上腺素+100mg/kg/天)是构成的六(6)组。使用组织病理学和免疫组织化学方法分析组织,而血清样本使用ELISA方法进行分析。
结果:MI组的组织病理学研究结果显示,观察到炎症细胞增加,拥塞,肺泡间隔增厚,红细胞加载巨噬细胞和肺组织纤维化。然而,处理组记录了关于这些参数的显著差异。在免疫组织化学分析中,在肺血管和细支气管的平滑肌结构和肺泡间区域中观察到asprosin和spexin的表达,还有细支气管上皮.在细支气管上皮中的asprosin和spexin表达方面,两组之间没有显着差异。当检测免疫组织化学和血清ELISA结果时,观察到,与对照组相比,MI组的肺组织中的asprosin水平显着增加,在MI后接受维生素D和NRD治疗的治疗组显著降低。与对照组相比,MI组的spexin显着降低,它在MI+V∞TD组中显著增加,但MI+NRD组中没有变化。
结论:观察到由于心肌梗塞导致肺部严重损伤,VITD和NRD治疗对这些损伤有疗效。还观察到Asprosin和Speksin蛋白可以对肺的损伤和治疗机制都有影响。此外,VITD的疗效取决于asprosin和spexin的表达;而观察结果表明,nerolidol可以通过asprosin依赖性机制和独立机制发挥作用。
Injury to internal organs caused by myocardial infarction (MI), although often neglected, is a very serious condition which damages internal organs especially the lungs. Changes in microcirculation can begin with acute lung injury and result in severe respiratory failure. The aim of this study was to create new approaches that will explain the pathophysiology and treatment of the disease by examining the therapeutic effects of vitamin D (VITD) and Nerolidol (NRD) on the injuries of the lungs caused by MI, and their relationship with asprosin / spexin proteins.
METHODS: Six groups of seven experimental animals each were constituted. Control, VITD (only 50 IU/day during the experiment), NRD (only 100 mg/kg/day during the experiment), MI (200 mg/kg isoproterenol was administered to rats as a single dose subcutaneously), MI+VITD (200 mg/kg isoproterenol +50 IU/day) and MI+NRD (200 mg/kg isoproterenol +100 mg/kg/day) were the six (6) groups constituted. Tissues were analyzed using histopathological and immunohistochemical methods, whereas serum samples were analyzed using ELISA method.
RESULTS: The result of the histopathological study for the MI group showed an observed increase in inflammatory cells, congestion, interalveolar septal thickening, erythrocyteloaded macrophages and fibrosis in the lung tissues. The treatment groups however recorded significant differences with regards to these parameters. In the immunohistochemical analysis, expressions of asprosin and spexin were observed in the smooth muscle structures and interalveolar areas of the vessels and bronchioles of the lung, as well as the bronchiole epithelium. There was no significant difference between the groups in terms of asprosin and spexin expression in the bronchiol epithelium. When immunohistochemical and serum ELISA results were examined, it was observed that asprosin levels increased significantly in the lung tissues of the MI group compared to the control group, decreased significantly in the treatment groups treated with Vitamin D and NRD after MI. While spexin decreased significantly in the MI group compared to the control group, it increased significantly in the MI+VİTD group, but did not change in the MI+NRD group.
CONCLUSIONS: It was observed that serious injuries occurred in the lungs due to myocardial infarction and that, VITD and NRD treatments had a curative effect on those injuries. It was also observed that Asprosin and Speksin proteins can have effect on mechanisms of both injury and therapy of the lung. Furthermore, the curative effects of VITD are dependent on the expression of asprosin and spexin; whereas the observation indicated that nerolidol could be effective through asprosin-dependent mechanisms and specisin by independent mechanisms.