在本研究中,采用BGISQ-500RNA-Seq技术研究了Scyllaparamamosain如何在分子水平上适应盐度耐受性,并探索了暴露于低盐度(5‰)和标准盐度(23‰)条件后与盐度适应相关的基因表达变化。共鉴定出1100个和520个差异表达基因(DEGs)在前、后背,分别,并在火山地块和热图中可视化了它们相应的表达模式。进一步的分析强调了已建立的基因功能类别和信号通路的显着富集,包括那些与细胞应激反应相关的,离子传输,能量代谢,氨基酸代谢,H2O传输,和生理压力补偿。我们还选择了编码关键应力适应和耐受性调节剂的前g和后g内的关键DEG,包括AQP,ABCA1,HSP10,A35,CAg,NKA,VPA,CAc,SPS。有趣的是,g中的A35可能通过结合CHH来调节渗透压,以响应低盐度胁迫或作为能量补偿的机制。一起来看,我们的发现阐明了S.paramamosain用于盐度适应的复杂分子机制,这涉及前和后g中不同的基因表达模式。这些发现为随后对盐度响应的候选基因的研究提供了立足点,并有助于更深入地了解Paramamosain在低盐度环境中的适应机制。这对于发展低盐度物种栽培和建立稳健的栽培模式至关重要。
In the present study, BGISEQ-500 RNA-Seq technology was adopted to investigate how Scylla paramamosain adapts to salinity tolerance at the molecular level and explores changes in gene expression linked to salinity adaptation following exposure to both low salinity (5 ‰) and standard salinity (23 ‰) conditions. A total of 1100 and 520 differentially expressed genes (DEGs) were identified in the anterior and posterior gills, respectively, and their corresponding expression patterns were visualized in volcano plots and a heatmap. Further analysis highlighted significant enrichment of well-established gene functional categories and signaling pathways, including those what associated with cellular stress response, ion transport, energy metabolism, amino acid metabolism, H2O transport, and physiological stress compensation. We also selected key DEGs within the anterior and posterior gills that encode pivotal stress adaptation and tolerance modulators, including AQP, ABCA1, HSP 10, A35, CAg, NKA, VPA, CAc, and SPS. Interestingly, A35 in the gills might regulate osmolality by binding CHH in response to low salinity stress or serve as a mechanism for energy compensation. Taken together, our findings elucidated the intricate molecular mechanism employed by S. paramamosain for salinity adaptation, which involved distinct gene expression patterns in the anterior and posterior gills. These findings provide the foothold for subsequent investigations into salinity-responsive candidate genes and contribute to a deeper understanding of S. paramamosain\'s adaptation mechanisms in low-salinity surroundings, which is crucial for the development of low-salinity species cultivation and the establishment of a robust culture model.