PDF(Pubmed)
Abstract：
Sepia esculenta is an economically important mollusk distributed in the coastal waters of China. Juveniles are more susceptible to stimulation by the external environment than mature individuals. The ocean salinity fluctuates due to environmental changes. However, there is a lack of research on the salinity adaptations of S. esculenta. Therefore, in this study, we investigated the differential expression of genes in S. esculenta larvae after stimulation by low salinity. RNA samples were sequenced and 1039 differentially expressed genes (DEGs) were identified. Then, enrichment analysis was performed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Finally, a protein-protein interaction network (PPI) was constructed, and the functions of key genes in S. esculenta larvae after low-salinity stimulation were explored. We suggest that low salinity leads to an excess proliferation of cells in S. esculenta larvae that, in turn, affects normal physiological activities. The results of this study can aid in the artificial incubation of S. esculenta and reduce the mortality of larvae.
摘要：
乌贼是分布在中国沿海水域的重要经济软体动物。青少年比成熟个体更容易受到外界环境的刺激。海洋盐度因环境变化而波动。然而,缺乏对esculenta的盐度适应的研究。因此,在这项研究中，我们研究了低盐度刺激后S.esculenta幼虫中基因的差异表达。对RNA样品进行测序并鉴定1039个差异表达基因(DEG)。然后，使用基因本体论(GO)和京都基因和基因组百科全书(KEGG)数据库进行富集分析。最后,构建了蛋白质-蛋白质相互作用网络(PPI)，探讨了低盐度刺激后esculenta幼虫关键基因的功能。我们建议低盐度会导致esculenta幼虫中细胞的过度增殖，反过来,影响正常的生理活动。这项研究的结果可以帮助S。esculenta的人工孵化并降低幼虫的死亡率。
