Abstract：
Transcriptome sequencing has offered immense opportunities to study non-model organisms. Abalone is an important marine mollusk that encounters harsh environmental conditions in its natural habitat and under aquaculture conditions; hence, research that increases molecular information to understand abalone physiology and stress response is noteworthy. Accordingly, the study used transcriptome sequencing of the gill tissues of abalone exposed to low salinity stress. The aim is to explore some enriched pathways during salinity stress and the crosstalk and functions of the genes involved in the candidate biological processes for future further analysis of their expression patterns. The data suggest that abalone genes such as YAP/TAZ, Myc, Nkd, and Axin (involved in the Hippo signaling pathway) and PI3K/Akt, SHC, and RTK (involved in the Ras signaling pathways) might mediate growth and development. Thus, deregulation of the Hippo and Ras pathways by salinity stress could be a possible mechanism by which unfavorable salinities influence growth in abalone. Furthermore, PEPCK, GYS, and PLC genes (mediating the Glucagon signaling pathway) might be necessary for glucose homeostasis, reproduction, and abalone meat sensory qualities; hence, a need to investigate how they might be influenced by environmental stress. Genes such as MYD88, IRAK1/4, JNK, AP-1, and TRAF6 (mediating the MAPK signaling pathway) could be useful in understanding abalone\'s innate immune response to environmental stresses. Finally, the aminoacyl-tRNA biosynthesis pathway hints at the mechanism by which new raw materials for protein biosynthesis are mobilized for physiological processes and how abalone might respond to this process during salinity stress. Low salinity clearly regulated genes in these pathways in a time-dependent manner, as hinted by the heat maps. In the future, qRT-PCR verification and in-depth study of the various genes and proteins discussed would provide enormous molecular information resources for the abalone biology.
摘要：
转录组测序为研究非模型生物提供了巨大的机会。鲍鱼是一种重要的海洋软体动物，在其自然栖息地和水产养殖条件下遇到恶劣的环境条件；因此，增加分子信息以了解鲍鱼生理和应激反应的研究值得注意。因此，这项研究使用了暴露于低盐度胁迫的鲍鱼ill组织的转录组测序。目的是探索盐度胁迫期间的一些富集途径以及参与候选生物过程的基因的串扰和功能，以便将来进一步分析其表达模式。数据表明，鲍鱼基因如YAP/TAZ，Myc,Nkd,和Axin（参与Hippo信号通路）和PI3K/Akt，SHC,和RTK（参与Ras信号通路）可能介导生长和发育。因此，盐度胁迫对Hippo和Ras途径的失调可能是不利的盐度影响鲍鱼生长的可能机制。此外，PEPCK,GYS,和PLC基因（介导胰高血糖素信号通路）可能是葡萄糖稳态所必需的，繁殖，和鲍鱼肉的感官品质；因此，需要调查它们如何受到环境压力的影响。基因如MYD88,IRAK1/4,JNK,AP-1和TRAF6（介导MAPK信号通路）可能有助于了解鲍鱼对环境压力的先天免疫反应。最后,氨酰-tRNA生物合成途径提示了蛋白质生物合成的新原料被动员用于生理过程的机制，以及鲍鱼在盐度胁迫期间如何应对这一过程。低盐度以时间依赖的方式明显调节这些途径中的基因，正如热图所暗示的那样。在未来,qRT-PCR验证和对所讨论的各种基因和蛋白质的深入研究将为鲍鱼生物学提供巨大的分子信息资源。
