目的:心血管疾病(CVD)的预防措施已将重点从脂蛋白转移到了免疫系统。然而,低度炎症和血脂异常紧密纠缠在一起。这项研究的目的是评估一组广泛的炎症生物标志物与脂蛋白亚类参数之间的关系。
方法:我们利用了基于人群的波美拉尼亚健康研究的数据(SHIP-TREND,n=403)。通过基于珠的测定测量37种炎性标志物的血浆浓度。此外,我们使用核磁共振波谱来测量总胆固醇,总甘油三酯,总磷脂以及胆固醇的分数浓度,甘油三酯,磷脂,ApoA1,ApoA2和ApoB在所有主要的脂蛋白亚类中。通过调整的线性回归模型分析炎症生物标志物和脂蛋白亚类之间的关联。
结果:4月,BAFF,TWEAK,sCD30,Pentraxin-3,sTNFR1,sTNFR2,骨钙蛋白,几丁质酶3样1,IFN-α2,IFN-γ,IL-11、IL-12p40、IL-29、IL-32、IL-35、TSLP、MMP1和MMP2与脂蛋白亚类成分有关,形成两个不同的集群。APRIL与HDL-C(总和亚类)以及HDLApo-A1和Apo-A2含量呈负相关。MMP-2与VLDL-C(总和亚类)呈负相关,IDL-C以及LDL5/6-C和VLDL-TG,IDL-TG,总甘油三酯以及LDL5/5-TG和HDL4-TG。此外,我们发现了一组与Th1免疫反应相关的细胞因子,与动脉粥样硬化脂蛋白有关。
结论:我们的发现扩展了对炎症-脂蛋白相互作用的现有知识,其中许多被认为与慢性非传染性疾病的病因有关。我们的研究结果支持使用免疫调节物质治疗和可能预防CVD。
OBJECTIVE: Prevention measures for cardiovascular diseases (CVD) have shifted their focus from lipoproteins to the immune system. However, low-grade inflammation and dyslipidemia are tightly entangled. The objective of this study was to assess the relations between a broad panel of inflammatory biomarkers and lipoprotein subclass parameters.
METHODS: We utilized data from the population-based Study of Health in Pomerania (SHIP-TREND, n = 403). Plasma concentrations of 37 inflammatory markers were measured by a bead-based assay. Furthermore, we employed nuclear magnetic resonance spectroscopy to measure total cholesterol, total triglycerides, total phospholipids as well as the fractional concentrations of cholesterol, triglycerides, phospholipids, ApoA1, ApoA2 and ApoB in all major lipoprotein subclasses. Associations between inflammatory biomarkers and lipoprotein subclasses were analyzed by adjusted linear regression models.
RESULTS: APRIL, BAFF, TWEAK, sCD30, Pentraxin-3, sTNFR1, sTNFR2, Osteocalcin, Chitinase 3-like 1, IFN-alpha2, IFN-gamma, IL-11, IL-12p40, IL-29, IL-32, IL-35, TSLP, MMP1 and MMP2 were related with lipoprotein subclass components, forming two distinct clusters. APRIL had inverse relations to HDL-C (total and subclasses) and HDL Apo-A1 and Apo-A2 content. MMP-2 was inversely related to VLDL-C (total and subclasses), IDL-C as well as LDL5/6-C and VLDL-TG, IDL-TG, total triglycerides as well as LDL5/5-TG and HDL4-TG. Additionally, we identified a cluster of cytokines linked to the Th1-immune response, which were associated with an atherogenic lipoprotein profile.
CONCLUSIONS: Our findings expand the existing knowledge of inflammation-lipoprotein interactions, many of which are suggested to be involved in the pathogeneses of chronic non-communicable diseases. The results of our study support the use of immunomodulatory substances for the treatment and possibly prevention of CVD.