:cerasus)是一种传统上用于改善慢性疾病的替代药物,其特征是氧化应激如神经病变。据报道,氧化应激控制可以改善炎症过程。本研究旨在利用体内生物模型进行植物化学研究。抗炎,和抗伤害感受潜能及其可能的作用机制。用EtAc进行超声处理以提取紫苏果(Scf),和种子(Scs)。通过RP-HPLC进行Scf的植物化学研究,而Scs的研究是利用GC-FID进行的。利用柱色谱法进行了生物引导馏分和分离方法,并且已经表明花青素-3-葡萄糖苷(Cy3G)是Scf中最活跃的成分,而亚油酸(LA)是Scs中最活跃的成分。Scf,Scs,Cy3G,LA显着(p=0.05)保护胃粘膜免受HCl/EtOH诱导的胃病变。Scs(200mg/kg)显示出最有可能的胃保护作用,结果与雷尼替丁(50mg/kg)相当。Scf,Scs,Cy3G,LA对角叉菜胶诱导的水肿和伤害性疼痛显示出显著的抗炎和抗伤害的潜力,分别,其中Scs(200mg/kg)显示出最大的抗炎和镇痛潜力,结果与布洛芬(100mg/kg)相当。Scf,Scs,Cy3G,和LA有对抗角叉菜胶诱导的氧化应激标志物,血清过氧化氢酶和还原型谷胱甘肽水平升高,和减少脂质过氧化。组织病理学研究证明了胃保护潜力,用最高剂量的Scs和Scf处理时,脾脏结构的再生和改善。降低促炎TNF-α和IL-6,升高抗炎因子IL-10水平,脾脏再生能力和氧化应激的改善可能是主要的机制。P.cerasus似乎有助于改善炎症过程,并在保留胃的同时降低疼痛阈值。
Prunus cerasus (P. cerasus) is an alternative-medicine used traditionally for amelioration of chronic-ailments marked by elevation in oxidative-stress like neuropathy. The oxidative-stress control was reported to ameliorate the inflammatory-process. This study aimed to phytochemically-investigate P. cerasus most-active phytochemicals utilizing in-vivo biological models to explore their gastroprotective, anti-inflammatory, and antinociceptive potentials and their possible mechanisms of action. Sonication with EtAc was used to extract P. cerasus fruit (Scf), and seed (Scs). The phytochemical-investigation of Scf was performed by RP-HPLC, while that of Scs was explored utilizing GC-FID. A bio-guided-fraction and isolation method was done utilizing column-chromatography, and have shown that cyanidin-3-glucoside (Cy3G) was the most-active constituent in Scf, while linoleic-acid (LA) was the most-active constituent in Scs. Scf, Scs, Cy3G, and LA significantly (p ˂ 0.05) protected the gastric-mucosa against HCl/EtOH-induced gastric-lesions. Scs (200 mg/kg) has shown the most gastroprotective-potentials, and had comparable-results to ranitidine (50 mg/kg). Scf, Scs, Cy3G, and LA have shown significant anti-inflammatory and antinociceptive potentials against carrageenan induced-edema and nociceptive-pain, respectively, where Scs (200 mg/kg) has shown the most anti-inflammatory and antinociceptive potentials, and had comparable results to ibuprofen (100 mg/kg). Scf, Scs, Cy3G, and LA have counter-acted carrageenan-induced oxidative-stress markers, with increased serum-catalase and reduced-glutathione levels, and decreased lipid-peroxidation. Histopathological-studies demonstrated gastroprotective potentials, regeneration and improvement of the spleen-structural architecture when treated with highest doses of Scs and Scf. The reduction of the pro-inflammatory TNF-alpha and IL-6, and elevation the anti-inflammatory factor IL-10 levels, spleen regenerative-capacity and oxidative-stress amelioration might be the main-mechanism responsible for P. cerasus anti-inflammatory potentials. P. cerasus appears to aid in ameliorating the inflammatory process, and reducing pain-thresholds while preserving the stomach.