背景:掌plant脓疱病(PPP)和pompholix是慢性疾病,其特征是手掌和脚掌上的脓疱和囊泡。这些疾病通常具有相似的临床病理特征,导致诊断困难。我们旨在使用免疫组织化学染色研究PPP和pompholix中角蛋白和总蛋白的表达模式。
方法:对来自PPP(n=40)和蓬松(n=22)患者的皮肤活检组织进行了角蛋白5、9、14和总蛋白表达的免疫组织化学分析。
结果:K5在PPP中的表达高于在pompholix中的表达,在基底有弥漫性阳性表达,棘突,和粒状层。K14表达在组间没有差异。K9表达在绒球囊泡(P=0.014)和棘层(P<0.001)附近观察到,但在PPP脓疱周围几乎没有。缠结蛋白的表达散布在PPP脓疱周围,部分散布在pompholyx囊泡周围,但无统计学意义(P=0.123)。PPP基底层中的Involucrin表达高于pompholix(P=0.023)。
结论:PPP和pompholyx在K5,K9和包膜蛋白的表达中表现出独特的分化。
BACKGROUND: Palmoplantar pustulosis (PPP) and pompholyx are chronic diseases characterized by pustules and vesicles on the palms and soles. These disorders often have similar clinicopathological features, which lead to diagnostic difficulties. We aimed to investigate the expression patterns of keratins and involucrin in PPP and pompholyx using immunohistochemical staining.
METHODS: Skin biopsies from patients with PPP (n = 40) and pompholyx (n = 22) were immunohistochemically analyzed for Keratin 5, 9, 14, and involucrin expression.
RESULTS: K5 expression was higher in PPP than in pompholyx, with diffusely positive expression in the basal, spinous, and granular layers. K14 expression did not differ between groups. K9 expression was observed near the pompholyx vesicle (P = 0.014) and stratum spinosum (P < 0.001) but was almost absent around PPP pustules. Involucrin expression was diffused around the PPP pustules and partially around the pompholyx vesicles, but without statistical significance (P = 0.123). Involucrin expression was elevated in the basal layer of the PPP compared with that in the pompholyx (P = 0.023).
CONCLUSIONS: PPP and pompholyx exhibited distinctive differentiation in the expression of K5, K9, and involucrin.