背景:衣康酸是一种用于合成聚合物的有前途的生物基结构单元,塑料,纤维和其他材料。近年来,Ustilagocynodontis已经成为一种额外的生产衣康酸的非常规酵母,主要是由于其高耐酸性,这大大减少了发酵和下游加工过程中盐渍废物的共同产生。因此,这可能会提高乌草科衣康酸生产过程的经济可行性。
结果:在这项研究中,我们在受控补料分批发酵中表征了先前工程化的高产衣康酸UstilagoCynodontis菌株,以确定衣康酸生产的最低和最佳pH值。在最佳发酵条件下,在pH3.6的发酵中,高产菌株在生产阶段可以达到理论上最大的衣康酸酯产量,但是以相当多的碱添加为代价。在pH值为2.8时,碱消耗大大降低,但以生产成本为代价,滴度,和率。基于整个过程的技术经济分析表明,由于pH控制试剂和盐水废物成本的额外降低而产生的节省不能补偿在高酸性pH值2.8下观察到的产量损失。
结论:总体而言,这项工作提供了关于产量平衡的新数据,滴度,在pH值的情况下,从而有助于更好地了解乌斯拉戈囊炎的衣康酸生产过程,尤其是从经济角度来看。
BACKGROUND: Itaconic acid is a promising bio-based building block for the synthesis of polymers, plastics, fibers and other materials. In recent years, Ustilago cynodontis has emerged as an additional itaconate producing non-conventional yeast, mainly due to its high acid tolerance, which significantly reduces saline waste coproduction during fermentation and downstream processing. As a result, this could likely improve the economic viability of the itaconic acid production process with Ustilaginaceae.
RESULTS: In this study, we characterized a previously engineered itaconate hyper-producing Ustilago cynodontis strain in controlled fed-batch fermentations to determine the minimal and optimal pH for itaconate production. Under optimal fermentation conditions, the hyper-producing strain can achieve the theoretical maximal itaconate yield during the production phase in a fermentation at pH 3.6, but at the expense of considerable base addition. Base consumption is strongly reduced at the pH of 2.8, but at cost of production yield, titer, and rate. A techno-economic analysis based on the entire process demonstrated that savings due to an additional decrease in pH control reagents and saline waste costs cannot compensate the yield loss observed at the highly acidic pH value 2.8.
CONCLUSIONS: Overall, this work provides novel data regarding the balancing of yield, titer, and rate in the context of pH, thereby contributing to a better understanding of the itaconic acid production process with Ustilago cynodontis, especially from an economic perspective.