Indocyanine green fluorescence imaging

吲哚菁绿荧光成像
  • 文章类型: Letter
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  • 文章类型: Journal Article
    在回顾性研究中,如果将血运重建针对向伤口血管体提供动脉血流的小腿动脉,则伤口愈合和腿部抢救效果更好。没有关于血运重建如何改变足血管小体血流量的数据。这项研究的目的是评估所有足血管体的股下动脉血运重建后灌注的变化,并比较直接血运重建(DR)血管体与间接血运重建(IR)血管体。
    在这项前瞻性研究中,在手术或血管内膝关节下血运重建术之前和之后,使用吲哚菁绿荧光成像(ICG-FI)测量足部灌注。根据血管造影,我们将足血管体分为DR和IR血管体。此外,在子分析中,如果血管再血管化的动脉产生了强烈的络脉,则IR血管体被分级为IR_Coll血管体,如果没有看到强烈的络脉,则作为IR_Coll-血管体。
    总共72英尺(28个旁路,分析了44个血管内血运重建)和282个血管体。手术和血管内血运重建术显著增加DR和IR血管体的灌注。搭桥手术后,IR血管体DR血管体的增加分别为55U和53U;IR和DR血管体之间的灌注增加没有显著差异.血管内血运重建后,灌注明显增加,40U,与IR血管体中的26U相比(p<0.05)。在IR血管体的亚分析中,无论是否存在强大的络脉,手术旁路后灌注均显着增加。血管内血运重建后,然而,在IR_Coll+但IR_Coll-亚组没有发现显著的灌注增加。
    开放血管重建术同样增加DR和IR血管体的灌注,而血管内血运重建增加DR的灌注显著高于IR血管体。强大的侧支网络可能有助于增加IR血管体的灌注。
    UNASSIGNED: In retrospective studies, wound healing and leg salvage have been better if revascularization is targeted to the crural artery supplying arterial flow to the wound angiosome. No data exist on how revascularization changes the blood flow in foot angiosomes. The aim of this study was to evaluate the change in perfusion after infrapopliteal artery revascularization in all foot angiosomes and to compare directly revascularized (DR) angiosomes to the indirectly revascularized (IR) angiosomes.
    UNASSIGNED: In this prospective study, foot perfusion was measured with indocyanine green fluorescence imaging (ICG-FI) before and after either surgical or endovascular below-knee revascularization. According to angiograms, we divided the foot angiosomes into DR and IR angiosomes. Furthermore, in a subanalysis, the IR angiosomes were graded as IR_Coll+ angiosomes if there were strong collaterals arising from the artery which was revascularized, and as IR_Coll- angiosomes if strong collaterals were not seen.
    UNASSIGNED: A total of 72 feet (28 bypass, 44 endovascular revascularizations) and 282 angiosomes were analyzed. Surgical and endovascular revascularization increased perfusion significantly in both DR and IR angiosomes. After bypass surgery, the increase in DR angiosomes was 55 U and 53 U in IR angiosomes; there were no significant difference in the perfusion increase between IR and DR angiosomes. After endovascular revascularization, perfusion increased significantly more, 40 U, in DR angiosomes compared to 26 U in IR angiosomes (p < 0.05). In the subanalysis of IR angiosomes, perfusion increased significantly after surgical bypass regardless of whether strong collaterals were present or not. After endovascular revascularization, however, a significant perfusion increase was noted in the IR_Coll+ but not in the IR_Coll- subgroup.
    UNASSIGNED: Open revascularization increased perfusion equally in DR and IR angiosomes, whereas endovascular revascularization increased perfusion significantly more in DR than in IR angiosomes. Strong collateral network may help increase perfusion in IR angiosomes.
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  • 文章类型: Journal Article
    背景:腹腔镜左半肝切除术(LLH)已被证明是治疗主要影响左半肝的肝胆管结石的有效且安全的方法。然而,这一程序仍然存在挑战。由于肝内胆管结石的病理变化,安全解剖肺门血管并确定精确的切除边界仍然很困难,即使有荧光成像。我们的团队提出了一种新的增强现实导航(ARN)结合吲哚菁绿(ICG)荧光成像的方法,用于肝胆管结石病例的LLH。本研究旨在探讨这种联合方法在手术中的可行性。
    方法:在2021年5月至2023年9月之间,纳入了16例接受LLH的肝胆管结石患者。所有患者均接受术前3D评估,然后在手术过程中使用ARN和ICG荧光成像进行指导。评估围手术期和术后短期结果,以评估该方法的安全性和有效性。
    结果:所有16例患者均成功接受了LLH。平均手术时间380.31±92.17min,平均估计失血量为116.25±64.49ml。ARN成功地帮助指导所有患者的肺门血管夹层。ICG荧光成像成功识别了11例患者(68.8%)的肝切除边界。在其余5例(31.3%)荧光成像失败的患者中,虚拟肝段投影(VLSP)成功识别其切除边界。所有患者均未发生重大并发症。即时残石率,结石复发率,经T管窦道取石率为12.5%,6.3%,和6.3%,分别。
    结论:ARN和ICG荧光成像的结合提高了LLH治疗肝胆管结石的安全性和准确性。此外,在ICG荧光成像失败的情况下,ARN可以作为识别精确切除边界的安全有效工具。
    BACKGROUND: Laparoscopic left hemihepatectomy (LLH) has been shown to be an effective and safe method for treating hepatolithiasis primarily affecting the left hemiliver. However, this procedure still presents challenges. Due to pathological changes in intrahepatic duct stones, safely dissecting the hilar vessels and determining precise resection boundaries remains difficult, even with fluorescent imaging. Our team proposed a new method of augmented reality navigation (ARN) combined with Indocyanine green (ICG) fluorescence imaging for LLH in hepatolithiasis cases. This study aimed to investigate the feasibility of this combined approach in the procedure.
    METHODS: Between May 2021 and September 2023, 16 patients with hepatolithiasis who underwent LLH were included. All patients underwent preoperative 3D evaluation and were then guided using ARN and ICG fluorescence imaging during the procedure. Perioperative and short-term postoperative outcomes were assessed to evaluate the safety and efficacy of the method.
    RESULTS: All 16 patients successfully underwent LLH. The mean operation time was 380.31 ± 92.17 min, with a mean estimated blood loss of 116.25 ± 64.49 ml. ARN successfully aided in guiding hilar vessel dissection in all patients. ICG fluorescence imaging successfully identified liver resection boundaries in 11 patients (68.8%). In the remaining 5 patients (31.3%) where fluorescence imaging failed, virtual liver segment projection (VLSP) successfully identified their resection boundaries. No major complications occurred in any patients. Immediate stone residual rate, stone recurrence rate, and stone extraction rate through the T-tube sinus tract were 12.5%, 6.3%, and 6.3%, respectively.
    CONCLUSIONS: The combination of ARN and ICG fluorescence imaging enhances the safety and precision of LLH for hepatolithiasis. Moreover, ARN may serve as a safe and effective tool for identifying precise resection boundaries in cases where ICG fluorescence imaging fails.
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  • 文章类型: Journal Article
    由于严重的术后粘连和解剖变化,腹腔镜全胃切除术(LTG)治疗残余胃癌(RGC)需要先进的技术。我们使用术中吲哚菁绿(ICG)荧光成像对2例RGC患者进行了LTG。这两个病例先前都接受了远端胃切除术和Billroth-I重建胃癌,随后被诊断为残胃的早期胃癌。在手术期间静脉内施用吲哚菁绿(2.5mg/体)。手术期间使用近红外荧光腹腔镜检查清楚地看到肝脏和胆总管,并安全解剖肝胆器官和残胃之间的粘连。腹腔镜全胃切除术成功,无并发症,在这两种情况下,术后过程都很顺利。术中实时ICG荧光成像可清晰显示肝脏和胆总管,可用于LTG治疗严重粘连的RGC。
    Laparoscopic total gastrectomy (LTG) for remnant gastric cancer (RGC) requires advanced techniques due to severe postoperative adhesions and anatomic changes. We performed LTG in 2 patients with RGC using intraoperative indocyanine green (ICG) fluorescence imaging. Both cases previously underwent distal gastrectomy with Billroth-I reconstruction for gastric cancer and were subsequently diagnosed with early-stage gastric cancer of the remnant stomach. Indocyanine green (2.5 mg/body) was administered intravenously during surgery. The liver and common bile duct were clearly visualized during surgery using near-infrared fluorescence laparoscopy, and the adhesions between the hepatobiliary organs and remnant stomach were safely dissected. Laparoscopic total gastrectomy was successfully performed without complications, and the postoperative course was uneventful in both cases. Intraoperative real-time ICG fluorescence imaging allows clear visualization of the liver and common bile duct and can be useful in LTG for RGC with severe adhesions.
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  • 文章类型: Video-Audio Media
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  • 文章类型: Case Reports
    目的:气孔脱垂是造口后晚期常见的并发症。随着化疗的进展,双孔结肠造口术或回肠造口术和化疗用于治疗原发性不可切除的结直肠癌。进行双孔结肠造口术或回肠造口术的术前治疗,以帮助原发性结直肠癌的小型化。因此,未来造口脱垂的数量可能会增加。先前关于造口脱垂修复的报道集中在结肠造口术的单侧造口脱垂,没有关于结肠环形造口术或回肠造口术双侧造口脱出的报道。
    方法:我们报告了一种新的修复技术,该技术采用吲哚菁绿(ICG)荧光成像技术,采用吻合器改良Altemeier法,考虑了边缘动脉的分布,预防了边缘动脉损伤,具有重要的临床意义。
    结论:我们用于结肠环形造口术的口腔和肛门侧脱垂的新技术被认为是有效和安全的。
    OBJECTIVE: Stoma prolapse is a common complication in the late phase after stoma creation. With advances in chemotherapy, a double-orifice colostomy or ileostomy and chemotherapy are used to treat primary unresectable colorectal cancer. Preoperative therapy with a double-orifice colostomy or ileostomy is performed to aid primary colorectal cancer miniaturization. Therefore, the number of stoma prolapses will likely increase in the future. Previous reports on the repair of stoma prolapse focused on unilateral stoma prolapse of loop colostomy, and there are no reports about the bilateral stoma prolapse of loop colostomy or ileostomy.
    METHODS: We report a novel repair technique for oral and anal side (bilateral) stoma prolapse of a loop colostomy with the stapled modified Altemeier method using indocyanine green (ICG) fluorescence imaging considering the distribution of marginal artery in preventing marginal artery injury which has considerable clinical significance.
    CONCLUSIONS: Our novel technique for the oral and anal side prolapse of a loop colostomy is considered effective and safe.
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  • 文章类型: Journal Article
    微创手术在恶性疾病的治疗中越来越多。虽然食管切除术是一项困难的手术,有很长的学习曲线,实际上向腹腔镜/胸腔镜/机器人方法转变,由于可视化的优势,外科医生的舒适度(机器人手术)以及整个团队看到手术以及手术外科医生的可能性。尽管目前仍有许多有争议的话题,关于胃食管交界处(GOJ)腺癌患者的手术治疗,例如开放式或微创手术方法,食管胃切除的类型,淋巴结清扫的类型和其他,微创方法已被证明是一种减少切除术后并发症的方法,尤其是通过减少肺部并发症。新技术的实施使这种手术方法的适应症范围得以扩大。短期和长期的结果,以及对病人的好处-减少手术创伤,快速和容易的恢复-提供这种类型的手术治疗未来发展的前提。本文综述了GOJ腺癌微创治疗的最新进展和前景。
    Minimally invasive surgery is increasingly indicated in the management of malignant disease. Although oesophagectomy is a difficult operation, with a long learning curve, there is actually a shift towards the laparoscopic/thoracoscopic/ robotic approach, due to the advantages of visualization, surgeon comfort (robotic surgery) and the possibility of the whole team to see the operation as well as and the operating surgeon. Although currently there are still many controversial topics, about the surgical treatment of patients with gastro-oesophageal junction (GOJ) adenocarcinoma, such as the type of open or minimally invasive surgical approach, the type of oesophago-gastric resection, the type of lymph node dissection and others, the minimally invasive approach has proven to be a way to reduce postoperative complications of resection, especially by decreasing pulmonary complications. The implementation of new technologies allowed the widening of the range of indications for this type of surgical approach. The short-term and long-term results, as well as the benefits for the patient - reduced surgical trauma, quick and easy recovery - offer this type of surgical treatment the premises for future development. This article reviews the updates and perspectives on the minimally invasive approach for GOJ adenocarcinoma.
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  • 文章类型: Journal Article
    目的:胆道损伤是与肝脏手术相关的严重并发症。可以使用磁共振胰胆管造影术和X线胆管造影术评估肝内胆管解剖;然而,术中实时胆管可视化方法尚未报道.这项研究旨在证明通过胆道内吲哚菁绿与近红外腹腔镜检查在肝切除术中实时荧光胆道造影(FC)的可用性。
    方法:离体检测吲哚菁绿(ICG)溶液的最佳浓度。测量ICG溶液及其与胆汁的混合物的荧光强度。使用临床试验模型,将ICG溶液注入胆囊管,随后在肝切除术期间进行近红外腹腔镜检查。
    结果:用于FC的ICG溶液的最佳浓度为0.01至0.05mg/mL。在三例肝切除术中使用了三种不同的腹腔镜系统。在所有情况下,使用近红外腹腔镜系统可以清楚地观察到Glissonian鞘中的肝内胆管的荧光。一小块组织阻止了胆汁发光;因此,清除FC需要暴露Glissonian鞘。该过程还检测到肝脏切割表面的胆汁渗漏。
    结论:胆道ICG给药和近红外腹腔镜检查能够在大肝切除术期间实现实时肝内FC。
    OBJECTIVE: Biliary injury is a severe complication that can be associated with liver surgery. Intrahepatic biliary anatomy can be evaluated using magnetic resonance cholangiopancreatography and X-ray cholangiography; however, an intraoperative real-time bile duct visualization method has not yet been reported. This study aimed to demonstrate the availability of real-time fluorescent cholangiography (FC) by intrabiliary indocyanine green administration with near-infrared laparoscopy in major hepatectomy.
    METHODS: The optimal concentration of indocyanine green (ICG) solution was examined ex vivo. The fluorescence intensity of the ICG solution and its mixture with bile was measured. Using a clinical trial model, ICG solution was injected into the cystic duct, followed by near-infrared laparoscopy performed during hepatectomy.
    RESULTS: The optimal concentration of ICG solution for FC was between 0.01 and 0.05 mg/mL. Three different laparoscopic systems were used in three hepatectomy cases. In all cases, the fluorescence of the intrahepatic bile ducts in the Glissonian sheath was clearly visualized using the near-infrared laparoscopic system. A small piece of tissue prevented the bile glow; thus, exposure of the Glissonian sheath was necessary for clear FC. This procedure also detected bile leakage from the cut surface of the liver.
    CONCLUSIONS: Intrabiliary ICG administration and near-infrared laparoscopy enabled real-time intrahepatic FC during major hepatectomy.
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  • 文章类型: Journal Article
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  • 文章类型: Case Reports
    左上段切除术(LUDS)后的舌节扭转是一种罕见的并发症,原因尚不清楚。在这里,我们报告了一名患者在LUDS后因乳腺癌多发肺转移而发生舌节扭转的情况。一个肺结节位于S12段,另一个位于不完全叶间裂上的上叶和S6之间。使用带有吲哚菁绿荧光成像的视频辅助胸腔镜手术,通过扩展的LUDS切除肺转移。由于荧光平面和结节位置的非典型缺陷,舌段上的钉线相对于叶间线垂直倾斜。胸部X线和对比增强计算机断层扫描显示术后第二天舌节缺血和扭转,并完成舌节段切除术。此病例显示在LUDS期间垂直的段间吻合线是术后舌节扭转的重要原因之一。
    Lingular-segment torsion after left-upper division segmentectomy (LUDS) is a rare complication, and the cause remains unclear. Here we report the case of a patient who developed lingular-segment torsion after LUDS for multiple lung metastases of breast cancer. One lung nodule was located in the S1 + 2 segment and another between the upper lobe and S6 on an incomplete interlobar fissure. The lung metastases were resected by extended LUDS using video-assisted thoracic surgery with indocyanine green fluorescence imaging. The staple line on the lingular segment was vertically tilted against the interlobar line because of the atypical defect of the fluorescence plane and nodule position. Chest X-ray and contrast-enhanced computed tomography indicated ischemia and torsion of the lingular segment on the second postoperative day, and completion of lingular segmentectomy was performed. This case showed the vertical intersegment staple line during LUDS was one of the important causes of postoperative torsion of the lingular segment.
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