草鱼出血性疾病是由草鱼呼肠孤病毒(GCRV)引起的。该病传播迅速,致死率高,严重影响草鱼养殖。此外,草鱼出血性疾病的分子机制尚不清楚。为了破译GCRV对草鱼组织的影响,通过GCRV处理选择抗性草鱼A(GA)和易感草鱼B(GB),并建立了控制草鱼C(GC)。the,肝脏,通过组织学观察,在GCRV的影响下,肌肉组织表现出不同的起病症状。我们选择了具有显著症状差异的肌肉样品用于IlluminaRNA测序。使用基因本体论和京都基因和基因组百科全书的分析揭示了3512、3074和1853之间的差异表达基因GB,\"\"GCvs.GA,\"和\"GAvs.GB,\"分别。此外,鉴定了与干扰素(IFN)途径相关的40个差异免疫相关基因和28个差异干扰素刺激基因(ISGs)。GB和GA免疫源相关基因的表达,如MDA5,IL-34,NF-KB,TRIM25,SOCS3,CEBPB,和BCL2,以及与JAK-STAT信号通路相关的基因,如IRF4,STAT1,STAT3,JAK1和JAK2显著上调.IFN和JAK-STAT信号通路与抗GCRV感染密切相关。这项研究中的转录组数据和预测的免疫基因和ISG为GCRV的治疗提供了新的见解。
Grass carp hemorrhagic disease is caused by the grass carp reovirus (GCRV). The disease spreads rapidly and has a high fatality rate, which seriously affects grass carp culture. Moreover, the molecular mechanisms underlying grass carp hemorrhagic disease remain unclear. To decipher the effects of GCRV on grass carp tissues, resistant grass carp A (GA) and susceptible grass carp B (GB) were selected through GCRV treatment, and control grass carp C (GC) was also established. The gill, liver, and muscle tissues exhibited different onset symptoms under the influence of GCRV by histological observation. We selected muscle samples with significant differences in symptoms for Illumina RNA sequencing. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes revealed 3512, 3074, and 1853 differentially expressed genes between \"GC vs. GB,\" \"GC vs. GA,\" and \"GA vs. GB,\" respectively. Additionally, 40 differential immune-related genes and 28 differential interferon-stimulating genes (
ISGs) related to the interferon (IFN) pathway were identified. The expression of immunogene-related genes of GB and GA, such as MDA5, IL-34, NF-KB, TRIM25, SOCS3, CEBPB, and BCL2, and genes associated with the JAK-STAT signaling pathway, such as IRF4, STAT1, STAT3, JAK 1, and JAK 2, was significantly upregulated. The IFN and JAK-STAT signaling pathways were closely related to anti-GCRV infection. The transcriptome data and predicted immune genes and
ISGs in this study provide novel insights into the treatment of GCRV.