PDF(Pubmed)
Abstract：
We hypothesized that the hCG modulates the expression of IFNT-pathway and ISGs in bovine endometrium during early pregnancy. The aim of the current study is to evaluate the effect of hCG on IFNT-pathway signals and ISGs expression in endometrial cells. For this, 29 non-lactating cross-bread cows were used in the study and submitted to a 9-day fixed-time artificial insemination (FTAI) protocol. The day of the AI was considered Day 0 (D0), and five days (D5) after the FTAI, the cows were allocated into two groups: Control and hCG group, when a hCG group received a single dose of 2.500UI of hCG. On day 18 after FTAI (D18) cows were slaughtered and endometrial tissue samples were collected. There was no difference between the embryo recovery rate of the cows in C compared to the hCG. The hCG group increased the accessory corpus luteum formation rate. The hCG resulted in greater serum progesterone concentration in the hCG group compared to the C on Day 14. Only the expression of IFNAR2 and STAT1 were upregulated on pregnant cows of the hCG group compared to the C group. The pathway genes (JAK1, STAT2, and IRF9) were not regulated. The mRNA abundance of ISG15, MX1, MX2, and OAS1 was upregulated in pregnant cows for hCG group, compared to C group. The results show that the administration of hCG, 5 days after AI, in addition to increasing the serum progesterone, modulates the expression of IFNT-pathway and ISGs on bovine endometrium on Day 18 of pregnancy.
摘要：
我们假设hCG在妊娠早期调节牛子宫内膜中IFNT途径和ISG的表达。本研究的目的是评估hCG对子宫内膜细胞中IFNT途径信号和ISGs表达的影响。为此，在研究中使用了29头非泌乳交叉面包母牛，并接受了9天固定时间的人工授精（FTAI）方案。AI的日期被认为是第0天（D0），FTAI后五天(D5)，将奶牛分为两组：对照组和hCG组，当hCG组接受单剂量2.500UI的hCG时。在FTAI(D18)后第18天,屠宰奶牛并收集子宫内膜组织样品。C中奶牛的胚胎恢复率与hCG相比没有差异。hCG组增加了副黄体形成率。与第14天的C相比,hCG在hCG组中导致更高的血清孕酮浓度。与C组相比,仅IFNAR2和STAT1的表达在hCG组的妊娠母牛上上调。通路基因(JAK1、STAT2和IRF9)不受调控。hCG组妊娠奶牛中ISG15、MX1、MX2和OAS1的mRNA丰度上调,与C组相比。结果表明，HCG的给药，AI后5天，除了增加血清孕酮，在妊娠第18天调节牛子宫内膜上IFNT途径和ISGs的表达。
