UNASSIGNED:据报道,在荧光胆管造影期间吲哚菁绿(ICG)注射的最佳时机与ICG从血清中消散的时间之间存在显着差异。没有关于胆道液中ICG浓度随时间变化的报道。在这里,我们测量了ICG的浓度和胆总管(CBD)和肝脏之间的荧光强度比,这被认为是CBD能见度的参数。
未经授权:将ICG(0.05mg/kg)静脉注射到雌性猪(n=7)中。之后,在30分钟时检测到CBD和肝脏的荧光,2h,和4h。从插管的CBD管收集胆液。使用捕获的图像测量荧光强度并使用ImageJ图像处理程序计算。使用分光光度法测量ICG浓度,并使用方差分析检验进行比较。
未经证实:30分钟时的胆道ICG浓度,2h,4h为92.07±27.72μg/mL,37.14±9.76μg/mL(p<0.05vs.30分钟),和13.91±5.71μg/mL(p<0.05vs.30分钟),分别为p<0.01。30分钟时的CBD/肝脏荧光强度比,2h,和4h分别为1.25±0.72、2.39±1.28(p<0.05vs.30分钟和4小时),和3.38±1.73(p<0.05vs.30分钟和2小时),分别。
未经证实:30分钟时ICG胆汁浓度最高,而CBD/肝脏荧光强度比在4h时最高。降低肝脏的荧光强度可能是改善荧光胆管造影期间CBD可视化的重要方法。
未经批准:PE/EA/491-5/2020。
UNASSIGNED: A significant difference exists between the reported optimal timing of indocyanine green (ICG) injection during fluorescence cholangiography and ICG dissipation time from the serum. There are no reports on alterations in ICG concentration in biliary fluid over time. Herein, we measured the concentration of ICG and the fluorescence intensity ratio between the common bile duct (CBD) and liver, which was recognized as a parameter of the visibility of the CBD.
UNASSIGNED: ICG (0.05 mg/kg) was injected intravenously into female pigs (n = 7). Afterwards, the fluorescence of the CBD and liver was detected at 30 min, 2 h, and 4 h. Biliary fluid was collected from cannulated CBD tubes. The fluorescence intensity was measured using captured images and calculated using the ImageJ image-processing program. ICG concentration was measured using spectrophotometry and compared using an analysis of variance test.
UNASSIGNED: Biliary ICG concentrations at 30 min, 2 h, and 4 h were 92.07 ± 27.72 μg/mL, 37.14 ± 9.76 μg/mL (p < 0.05 vs. 30 min), and 13.91 ± 5.71 μg/mL (p < 0.05 vs. 30 min), respectively; p < 0.01. The CBD/liver fluorescence intensity ratios at 30 min, 2 h, and 4 h were 1.25 ± 0.72, 2.39 ± 1.28 (p < 0.05 vs. 30 min and 4 h), and 3.38 ± 1.73 (p < 0.05 vs. 30 min and 2 h), respectively.
UNASSIGNED: The ICG biliary concentration was highest at 30 min, whereas the CBD/liver fluorescence intensity ratio was highest at 4 h. Decreasing the fluorescence intensity of the liver may be an important approach for improving the visualization of the CBD during fluorescence cholangiography.
UNASSIGNED: PE/EA/491-5/2020.