PDF(Pubmed)
Abstract：
Protists are important key players in the microbial loop and influence their environment by grazing, which leads to the return of nutrients into the soil and reduces pathogen pressure on plants. Specifically, protists on and around plant roots are important for plants\' development and growth. For this study, the fourth most important crop in the world, Hordeum vulgare, was selected. Seeds of H. vulgare were inoculated with Acanthamoeba castellanii alone or with additional soil bacteria at the beginning and during the experiment. The germination of the seeds and the growth of the plants in pouches were monitored over 3 weeks. No differences were found in leaf growth, root growth, root and leaf nitrogen content or ammonia content of the liquid from the pouches. In contrast, the relative increase in root and leaf dry weight showed a small difference compared to the controls. The results of this experiment demonstrated that seed inoculation with A. castellanii alone or with additional unidentified soil bacteria did not have a major effect on the growth and development of barley. Nevertheless, small changes in plant development were detected, indicating that A. castellanii should be considered for further investigation of co-inoculations with plant growth-promoting bacteria and additional nutrients.
摘要：
原生生物是微生物循环中重要的关键参与者，通过放牧影响他们的环境，这导致养分返回土壤并减少病原体对植物的压力。具体来说,植物根部及其周围的原生生物对植物的发育和生长很重要。对于这项研究，世界上第四大重要作物，大麦，被选中。在实验开始和实验过程中，分别用棘阿米巴单独接种或与其他土壤细菌接种。在3周内监测种子的萌发和小袋中植物的生长。叶片生长没有差异，根系生长,袋中液体的根和叶氮含量或氨含量。相比之下,与对照相比，根和叶干重的相对增加显示出很小的差异。该实验的结果表明，单独接种A.castellanii或与其他未鉴定的土壤细菌一起接种种子对大麦的生长和发育没有重大影响。然而，检测到植物发育的微小变化，表明应考虑进一步研究与植物生长促进细菌和其他营养素的共同接种。
